Antibody Drug Development & Characterization
RayBiotech has a comprehensive catalog of products and services to advance drug discovery, from off-the-shelf arrays to screen for drug candidates to custom assays and services. Our comprehensive analytical services ensure compliance with CLIA, good clinical laboratory practices (GCLP), good laboratory practice (GLP), and current good manufacturing practice (cGMP) standards as well as current regulatory guidance documents based on industry requirements.
Antigen production and characterization
Protein-based drugs are usually synthetic versions of proteins that are produced naturally by the body. They modify cellular responses by either acting directly with the targets-of-interest, recruiting other molecules to the site, or improving the function of nearby cells. RayBiotech’s GMP-certified custom protein production encompasses every step of production from gene cloning through protein purification, and includes downstream characterization and quality control.
In vitro assay and screening
Characterize the interaction between the candidate antibody to the target molecule or cells to assess binding specificity, affinity and functional properties to identify and select for desired therapeutic effects
- ELISA
- Flow Cytometry
- Western Blotting
- Functional Assays
Epitope mapping
Epitope mapping is a method that is often employed to identify the antigen binding sites of an antibody. RayBiotech’s peptide array-based linear epitope mapping service uses high-quality peptides that are chemically synthesized.
- Small sample volume required (< 5 µL)
- Large dynamic range
- Suitable for high-throughput analyses
- High efficiency and accuracy
Antibody transient expression
Transient expression is a temporary and relatively quick method for antibody production, typically used for early-stage research, screening, or proof-of-concept studies. It allows for rapid generation of antibodies without the need for stable cell line generation. RayBiotech expertise can complete this process for you from DNA transfection to harvesting and characterization with fast turnaround.
Antibody stable cell line generation
RayBiotech's stable cell line service uses a mammalian retrovirus to incorporate foreign genes into the host genome. Our comprehensive service is customized to meet your needs.
Advantages of stable gene expression*
- Gene will be passed to daughter cells
- Cultured cells are genetically identical
- Large amounts of the protein/antibody-of-interest can be produced
- No antibiotic selection is required to maintain gene expression
* compared to transient gene expression
Antibody production and characterization
RayBiotech’s expert team can use your target antigen or peptide sequence of interest to develop a hybridoma and bulk monoclonal antibody production with scalable, large-scale production and purification services from pre-existing hybridoma cell lines to generate large quantities of a specific antibody. Serum-free culture, carrier-free, and less than 1 EU/mg endotoxin levels guaranteed. Characterization of these antibodies for in-depth understanding using the following analyses:
- Sensitivity
- Specificity
- Epitope Recognition
- Stability
- Immunogenicity
- In vivo studies
- Target engagement
- Neutralizing activity assessment
- Isotyping characterization
- Residual host protein analysis
- Mechanism of Action analysis
Vaccine Development
Antibody drugs can play a significant role in vaccine development. Utilize RayBiotech’s expertise and resources to
- Identify viral targets
- Characterize viral antigens
- Evaluation of vaccine efficacy
- Passive immunization
- Post-vaccination monitoring
