Flow Cytometry Service
RayBiotech provides a comprehensive Flow Cytometry Full Testing Service to evaluate cellular and signaling phenotypes, cell growth changes, cytokine production, cellular division, and even intracellular signaling events. Our flow cytometry team has over 25 years of experience, specializing in immunology (e.g., T cells) and stem cells.
Service Features:
- High quality analyses performed by experts
- Affordable
- Rapid turn-around
- Bulk discounts
- Long-term service contracts available
- GCLP certification of the marker panel available
What's Included:
- PDF report of scatter plots based on your gating strategy
- Excel report with cell counts
- Raw data file (available upon request at no additional fee)
Applications of Flow Cytometry Service:
- Immunophenotyping cells and exosomes based on intracellular and surface antigens
- Cell cycle analysis
- Detection of apoptotic cells
- Analysis of cell proliferation and activation
- Measuring proteins from protein extracts using RayPlex™ Bead Arrays
Additional Flow Cytometry Services
- Custom antibody conjugation to the fluorophore-of-interest
- Panel optimization
- Treating cells in cell culture
Flow Cytometry Fluorophores
| RayBright® Fluorophores | Exitation Laser | Max Exitation/Emission | Equivalent Fluorophores |
|---|---|---|---|
| RayBright® UV 450 | UV 355 | 347/448 nm | Alexa Fluor 350 |
| RayBright® Violet 450 | Violet 407 | 403/454 nm | Pacific Blue, Alexa Fluor 450 |
| RayBright® Violet 500 | 405/537 nm | Alexa Fluor 430, BV510 | |
| RayBright® Violet 600 | 407/600 nm | BD Brilliant Violet 605 | |
| RayBright® Violet 655 | 407/655 nm | BD Brilliant Violet 655 | |
| RayBright® Blue 488 | Blue 488 | 491/518 nm | Alexa Fluor 488, FITC, BD BB515 |
| RayBright® Blue/Green 575 | Blue 488/Green 561 | 505/570, 553/570 nm | R-PE, Cy3 |
| RayBright® Green 594 | Green 561 | 594/614 nm | PE-Texas Red, PE-CF594, PE-eFluor610 |
| RayBright® Blue/Green 647 (PE-tandem dye) | Blue 488/Green 561 | 496/664, 565/664 nm | PE-Cy5 |
| RayBright® Blue/Green 780 (PE-tandem dye) | Blue 488/Green 561 | 496/780, 565/780 nm | PE-Cy7, PE-Alexa Fluor 750 |
| RayBright® Red 647 | Red 640 | 649/664 nm | APC, Alexa Fluor 647, Cy5 |
| RayBright® Red 700 | 685/710 nm | Alexa Fluor 700, APC-R700 | |
| RayBright® Red 780 | 629/780 nm | Alexa Fluor 750, APC-eFluor 780 |
| Traditional Fluorophores | Exitation Laser | Max Exitation/Emission |
|---|---|---|
| PerCP | Blue 488 | 482/667 nm |
| R-PE | Blue 488/Green 561 | 496/780, 565/780 nm |
| APC | Red 640 | 651/662 nm |
Detection of specific T helper populations, including Th1, Th2, Th17, and Tregs
Discuss your project requirements
Frequently Asked Questions
Still have questions?
Fill out the service form and include it with your shipment to RayBiotech. Cells should be in the appropriate amount of dimethyl sulfoxide (DMSO) and shipped frozen (on dry ice).
See also our Sample Shipment Instructions.
- Aliquot the cells into cryovials containing cell culture media and dimethyl sulfoxide (DMSO) at the desired cell concentration. The appropriate amount of DMSO depends on the cells, but is usually 5 - 10% (v/v).
- Slowly freeze cells to maintain cell viability. You can either place the cryovials in a controlled rate freezing apparatus (decreasing the temperature approximately 1°C per minute) or in an isopropanol chamber overnight at -80°C.
- Store the cells in the gas phase in a liquid nitrogen tank.
- Ship the cells on dry ice. Ensure that there is enough dry ice to keep the cells frozen during shipping. Please ship your samples overnight on Mondays through Wednesdays only. Learn more about how to ship samples by clicking the SAMPLE SHIPMENT INSTRUCTIONS button below.
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