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| CODE | Number of Plates | Price | ||
|---|---|---|---|---|
|
ELHS-AGT-2
|
$1,197.00 | ||
|
|
ELHS-AGT-1
|
$665.00 | ||
|
|
ELHS-AGT-5
|
$2,660.00 |
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Assay Format
Number of Targets Detected:
1
Species Detected:
- Human
Design Principle:
- Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Protein Information
Accession Number:
- P01019
Gene ID:
- 183
Gene Symbols:
- AGT
- SERPINA8
Protein Name & Synonyms:
Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin-1 (Angiotensin 1-10) (Angiotensin I) (Ang I) Angiotensin-2 (Angiotensin 1-8) (Angiotensin II) (Ang II) Angiotensin-3 (Angiotensin 2-8) (Angiotensin III) (Ang III) (Des-Asp[1]-angiotensin II) Angiotensin-4 (Angiotensin 3-8) (Angiotensin IV) (Ang IV) Angiotensin 1-9 Angiotensin 1-7 Angiotensin 1-5 Angiotensin 1-4]
Product Features
- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Application Notes
Kit Components
- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 µl of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 µl of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
