Human Osteoactivin ELISA

RayBio® Human Osteoactivin (GPNMB) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $476.00
$380.80
You save: $95.20 (20%)
ELH-Osteoactivin-1
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Antigen Information

Accession Number:
  • Q14956
Gene ID:
  • 10457
Gene Symbols:
  • GPNMB
  • HGFIN
  • NMB
  • UNQ1725/PRO9925
Protein Name & Synonyms:
Transmembrane glycoprotein NMB (Transmembrane glycoprotein HGFIN)
Target Species:
  • Human

Assay Format

Detection Range:
45 pg/ml - 12,000 pg/ml
Recommended Dilution (serum & plasma):
10 - 110 fold
Sensitivity:
45 pg/ml

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Specificity:
This antibody pair detects human Osteoactivin. It show less than 20% cross-reactivity with recombinant mouse Osteoactivin.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
We especially appreciated how all the reagents were clearly labeled "A, B, C" etc. to minimize confusion. Worth it!

McLean Hospital
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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