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Custom Human p70 S6 Kinase IQELISA™ Kit

RayBio® Human Immunoquantitative (PCR-Based) p70 S6 Kinase ELISA Kit for cell culture supernatants, plasma, and serum samples. 7 week lead time.

$1,409.40  
IQH-P70S6K-2
+

Antigen Information

Accession Number:
  • P23443
Gene ID:
  • 6198
Gene Symbols:
  • RPS6KB1
  • STK14A
Protein Name & Synonyms:
Ribosomal protein S6 kinase beta-1 (S6K-beta-1) (S6K1) (EC 2.7.11.1) (70 kDa ribosomal protein S6 kinase 1) (P70S6K1) (p70-S6K 1) (Ribosomal protein S6 kinase I) (Serine/threonine-protein kinase 14A) (p70 ribosomal S6 kinase alpha) (p70 S6 kinase alpha) (p70 S6K-alpha) (p70 S6KA)
Target Species:
  • Human

Assay Format

Sensitivity:
Typically 10x lower than traditional ELISA
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
qPCR
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
NEED HELP RUNNING THIS ASSAY? Full testing service for only $5 More per plate REQUEST QUOTE | MORE INFO

Introduction

The RayBio® Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The RayBio® Custom Human p70 S6 Kinase IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human p70 S6 Kinase in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human p70 S6 Kinase coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and p70 S6 Kinase present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.

Typical Data

Application Notes

Kit Components
  • p70 S6 Kinase Microplate (Item A): 96 well PCR plate coated with anti-Human p70 S6 Kinase
  • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
  • Standards (Item C): 2 vials of recombinant Human p70 S6 Kinase
  • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
  • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
  • Detection Affinity Reagent for p70 S6 Kinase (Item F): 2 vials of a 4x concentrated solution of anti-Human p70 S6 Kinase affinity reagent
  • IQELISA™ Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
  • Primer Solution (Item I): 1.7 ml vial
  • PCR Master Mix (Item J): 1.2 ml vial
  • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
  • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
Other Materials Required
  • Real-time PCR instrument, Bio-Rad recommended
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Absorbent paper
  • Distilled or deionized water
  • Log-log graph paper or computer and software for data analysis
  • Tubes to prepare standard or sample dilutions
  • Heating block or water bath capable of 80°C
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed
  2. Add 25µl standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
  3. Add 25 µl detection affinity reagent to each well. Incubate 1 hour at room temperature
  4. Add 25µL of IQELISA™ Detection Reagent to each well. Incubate 1 hour
  5. Add 15µL Primer solution 10µL of PCR master mix to each well
  6. Run real-time PCR

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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