Mouse Periostin ELISA

$398.00
ELM-Periostin-1

RayBio® Mouse Periostin ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.

Product Features

Sensitivity
0.05 ng/ml

Looking for higher sensitivity? Check out our new Ultrasensitive Biomarker Testing Service powered by SIMOA™ technology.

Detection Range
0.05 ng/ml - 16 ng/ml
Recommended Dilution (serum & plasma)
50 - 500 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: Axl, BLC, CD27 Ligand, CD30, CD30 Ligand, CD40, CXCL16, EGF, E-selectin, Fractalkine, GITR, HGF, ICAM-1, IFN-gamma, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 ra, IL-1 beta, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-17E, IL-17F, IL-20, IL-23, IL-28, I-TAC, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, P-selectin, Resistin, SCF, SDF-1 alpha, TPO, VCAM-1.
Species Detected
Mouse
Protein Name & Synonyms
Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)
Accession Number
Gene Names
Postn, Osf2
Gene ID
50706

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • POSTN

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants
Write a review

No posts found

U.S. International

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Cart content Cart content