Mouse Renin 1 ELISA

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$461.60
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RayBio® Mouse Renin 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELM-Renin1-1
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Antigen Information

Accession Number:
  • P06281
Gene ID:
  • 19701
Gene Symbols:
  • REN
  • REN-1
  • REN1
Protein Name & Synonyms:
Renin-1 (EC 3.4.23.15) (Angiotensinogenase) (Kidney renin)
Target Species:
  • Mouse

Assay Format

Detection Range:
6 pg/ml - 50000 pg/ml
Recommended Dilution (serum & plasma):
2 - 20 fold
Sensitivity:
6 pg/ml

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Specificity:
This ELISA antibody pair detects mouse Renin. Other species not determined yet
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Have used this kit multiple times and am very satisfied with it. Great sensitivity for detection of renin in plasma and serum.

University of Iowa
Excellent dynamic range and sensitivity for hormone detection in serum with appropriate sample dilutions. Easy to use 8-well strip format, which makes it possible to run small sample batches on a single assay kit. Standards can be scaled to fit a smaller dynamic range for higher accuracy and reproducibility. As listed the protocol run-time is of average length for other ELISA products with an overnight incubation and wash steps. Colorimetric detection is quick and simple.
I have used a couple of the kits so far, and pretty much satisfied with my results.
But one of the problems I found was that I could never get the high concentration points (i.e. 50000pg/ml or 12500pg/ml) on the Standard Curve as your manual indicated, the machine was simply saturated. Also, I wish some of the reagents could last longer, not just for 5-7 days.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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