Mouse sTNFRI ELISA

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$321.60
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ELM-TNFR1-1

RayBio® Mouse TNF RI (TNFRSF1A) ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Antigen Information

Accession Number:
  • P25118
Gene ID:
  • 21937
Gene Symbols:
  • TNFR-1
  • TNFR1
  • TNFRSF1A
Protein Name & Synonyms:
Tumor necrosis factor receptor superfamily member 1A (Tumor necrosis factor receptor 1) (TNF-R1) (Tumor necrosis factor receptor type I) (TNF-RI) (TNFR-I) (p55) (p60) (CD antigen CD120a)
Target Species:
  • Mouse

Assay Format

Detection Range:
1 pg/ml - 300 pg/ml
Recommended Dilution (serum & plasma):
5 - 50 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA antibody pair detects mouse sTNFRI. Other species not determined.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
This ELISA kit is well designed and convenient to use. The plate is made of 12 8-strip wells that can be used for different experiment. The protocol is well written and easy to follow. In my experiment, it detected sTNFR1 in mouse serum in 1:5 and 1:10 dilution easily.
In the kit protocol, it calls for incubation time of 30min after TMB substrate addition. Each experiment varies but in most cases 30min is too long, it is recommended to monitor the developing every 5 minutes and stop the reaction at appropriate time point.

UT-Southwestern Medical Center
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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