Human Cytokine Array C5

The Cytokine Array C5 is a membrane-based antibody array for screening and comparing expression levels of 80 human cytokines in any liquid sample type.

$440.36  
AAH-CYT-5-2
+

Assay Format

Number of Targets Detected:
80
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ANG | BDNF | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18 | CCL2 | CCL20   View more

Introduction

The cytokine array is an antibody based immunoassay similar to ELISA, but able to simultaneously measure multiple proteins on one solid support. The Cytokine Array C5 is a membrane-based antibody array, using a panel of 80 different capture antibodies printed on a nitrocellulose membrane. The protein from the incubated sample is captured, and a detection antibody is also bound, creating the antibody-antigen-antibody sandwich. C-Series arrays are processed similarly to a western blot and the entire procedure can be completed in 1 day with little to no optimization.

Learn More About Cytokine Arrays

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
BDNF
BLC
(CXCL13)
CK beta 8-1
(CCL23)
EGF
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GM-CSF
GRO alpha
(CXCL1)
GRO alpha/beta/gamma
HGF
I-309
(TCA-3/CCL1)
IFN-gamma
IGF-1
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
IL-8
(CXCL8)
IL-10
IL-12 p40/p70
  • Gene Symbol: IL12A NKSF1
  • Gene ID: 3592
  • Accession #: P29459
IL-13
IL-15
IL-16
IP-10
(CXCL10)
Leptin
LIF
LIGHT
(TNFSF14)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
NAP-2
(PPBP/CXCL7)
NT-3
NT-4
Oncostatin M
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-BB
PLGF
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TGF beta 2
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
Thrombopoietin
(TPO)
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
VEGF-A

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C5 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Acharya C., Adesida A., Zajac P., et al. Enhanced chondrocyte proliferation and mesenchymal stromal cells chondrogenesis in coculture pellets mediate improved cartilage formation. J Cell Physiol. 2012 Jan;227(1):88-97. doi: 10.1002/jcp.22706.
    Species: Human
    Sample type: Conditioned Media (Chondrocyte growth model)
  2. He, Tongrong, et al. "Activation of Peroxisome proliferator-activated receptor–? enhances regenerative capacity of human endothelial progenitor cells by stimulating biosynthesis of tetrahydrobiopterin." Hypertension 58.2 (2011): 287-294.
    Species: Human
    Sample type: Conditioned Media (EPCs)
  3. Kikuta, S., Tanaka, N., Kazama, T., Kazama, M., Kano, K., Ryu, J., ... & Matsumoto, T. (2013). Osteogenic effects of dedifferentiated fat cell transplantation in rabbit models of bone defect and ovariectomy-induced osteoporosis. Tissue Engineering Part A, 19(15-16), 1792-1802.
    Species: Human
    Sample type: Conditioned Media (Human DFAT cells, ASCs, and bone marrow MSCs)
  4. Anderson-Baron, M., Kunze, M., Mulet-Sierra, A., Osswald, M., Ansari, K., Seikaly, H., & Adesida, A. B. (2020). Nasal chondrocyte derived soluble factors affect chondrogenesis of cocultured mesenchymal stem cells. Tissue Engineering Part A. doi:10.1089/ten.tea.2019.0306 
    Species: Human
    Sample type: Conditioned Media (Cell culture media)
  5. Wendt K, Wilk E, Buyny S, Buer J, Schmidt RE, Jacobs R. Gene and protein characteristics reflect functional diversity of CD56-dim and CD56-bright NK cells. J Leuk Biol. 2006;80:1529-1541.
    Species: Human
    Sample type: Conditioned Media (NK cell-conditioned media)
  6. Hashimoto I, Wada J, Hida A, Baba M. Elevated Serum Monoctye Chemoattractant Protein-4 and Chronic Inflammation in Overweight Subjects. Obesity. 2006:14:799-811.
    Species: Human
    Sample type: Serum (Serum)
  7. Ishiguro, Y., Ishiguro, H., & Miyamoto, H. (2013). Epidermal growth factor receptor tyrosine kinase inhibition up-regulates interleukin-6 in cancer cells and induces subsequent development of interstitial pneumonia. Oncotarget, 4(4), 550.
    Species: Human
    Sample type: Conditioned Media (HSC-3 or A549 cells treated with EGFR-TKI or ?EGFR antibody.)
  8. Velpula K., Dasari V., Rao J. The homing of human cord blood stem cells to sites of inflammation: unfolding mysteries of a novel therapeutic paradigm for glioblastoma multiforme. Cell Cycle. 2012 Jun 15;11(12):2303-13. doi: 10.4161/cc.20766.
    Species: Human
    Sample type: Conditioned Media (Umbilical Cord blood stem cell trafficking)
  9. Restall I., Parolin D., Daneshmand M., et al. PKC? depletion initiates mitotic slippage-induced senescence in glioblastoma. Cell Cycle. 2015;14(18):2938-48. doi: 10.1080/15384101.2015.1071744.
    Species: Human
    Sample type: Conditioned Media (Growing or senescent cell cultures)
  10. Henning R., Sanberg P., Jimenez. Human cord blood stem cell paracrine factors activate the survival protein kinase Akt and inhibit death protein kinases JNK and p38 in injured cardiomyocytes. Elsevier. [Publishing Information not found]
    Species: Human
    Sample type: Conditioned Media (Human huUCBC cells)
Works best for invitro mamalian cell cytokine assay

Forsyth Institute
I really liked this kit. It was very easy to use and came with detailed instructions as well troubleshooting tips. I have purchased another similar kit after using this. Highly recommend.

UNC Chapel Hill
The protocol is easy to follow. This kit only needs a small sample volume and can detect a low concentration of cytokine signaling.

University of Kansas
The Human Cytokine Array C5 kit gave me satisfied candidates under the conditioned medium. I strongly recommend this!!

Winship Cancer Institute of Emory University
easy to use and analysis, got excellent results!

NC State University
Simple to use and detects many cytokines

Johns Hopkins University School of Medicine
It works great using the cell growth media.

Emory University
I used on mammalian cell culture supernatant it and works well

Indiana University
Kit worked well with conditioned media from smooth muscle cells. Provides a lot of data in a small amount of time.

University of Nevada School of Medicine
I wanted to get something that can work on primary cell line and it turned out good. Results received after experiments had let us plan on which cytokine study we might need to work on for further study. Kit is simple and was of good quality.

University of Texas Health Science Center San Antonio
Easy to use, works well.

East Tennessee State University
Easy to use, and protocols are easy to follow. Recommended to fellow colleagues.

UCSF
Protocol is simple and worked great with our conditioned media!

UNMC
Simple and easy protocol with clean results.

Augusta University
The protocol suggested in the kit works very well for all arrays. Even if you need to optimize the time using the short exposition time suggested in the protocol, your array development will work great as usual!

Augusta University
Worked great with conditioned media. Followed the protocol in the kit and the results were super clean and of excellent quality.

Stanford University
it's an easy kit to use. However, I was not very impressed with their excel software. there was a mistake which we caught and ask them to fix bit they didn't even give us a discount. Other than it's straightforward and give you great results.

University of the pacific
Worked great for med-high abundant proteins. Customer service was spectacular

UCLA
This kit can detect huge amount of different cytokines and the price is only a little bit more than the kits that only test around 10 cytokines.

Nanjing Medical University
works good with condition media
Human Cytokine Array C5 kit is simple to use, has high quality and results are reproducible. Fast delivery from Raybiotech. But the value for the money is a little high.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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