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Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:

ANG | BDNF | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18 | CCL2 | CCL20 View more

Introduction

The cytokine array is an antibody based immunoassay similar to ELISA, but able to simultaneously measure multiple proteins on one solid support. The Cytokine Array C5 is a membrane-based antibody array, using a panel of 80 different capture antibodies printed on a nitrocellulose membrane. The protein from the incubated sample is captured, and a detection antibody is also bound, creating the antibody-antigen-antibody sandwich. C-Series arrays are processed similarly to a western blot and the entire procedure can be completed in 1 day with little to no optimization.

Learn More About Cytokine Arrays

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin Gene Symbol: ANG RNASE5 Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: RayBio Human BDNF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 BCA1 BLC SCYB13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA	CK beta 8-1 (CCL23) Gene Symbol: CCL23 MIP3 MPIF1 SCYA23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human Ckbeta8 ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA
ENA-78 (CXCL5) Gene Symbol: CXCL5 ENA78 SCYB5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 SCYA11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: CCL24 MPIF2 SCYA24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	Eotaxin-3 (CCL26) Gene Symbol: CCL26 SCYA26 UNQ216/PRO242 Gene ID: 10344 Accession #: Q9Y258 ELISA Kit: RayBio Human Eotaxin-3 ELISA	FGF-4 Gene Symbol: FGF4 HST HSTF1 KS3 Gene ID: 2249 Accession #: P08620 ELISA Kit: RayBio Human FGF-4 ELISA
FGF-6 Gene Symbol: FGF6 HST2 HSTF2 Gene ID: 2251 Accession #: P10767 ELISA Kit: RayBio Human FGF-6 ELISA	FGF-7 (KGF) Gene Symbol: FGF7 EGK_17479 Gene ID: 2252 Accession #: P21781 ELISA Kit: RayBio Human FGF-7 ELISA	FGF-9 Gene Symbol: FGF9 Gene ID: 2254 Accession #: P31371 ELISA Kit: RayBio Human FGF-9 ELISA	Flt-3 Ligand Gene Symbol: FLT3LG Gene ID: 2323 Accession #: P49771 ELISA Kit: RayBio Human Flt-3 L ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 FKN NTT SCYD1 A-152E5.2 Gene ID: 6376 Accession #: P78423 ELISA Kit: RayBio Human Fractalkine ELISA
GCP-2 (CXCL6) Gene Symbol: CXCL6 GCP2 SCYB6 Gene ID: 6372 Accession #: P80162 ELISA Kit: RayBio Human GCP-2 ELISA	GCSF Gene Symbol: CSF3 C17orf33 GCSF Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GDNF Gene Symbol: GDNF Gene ID: 2668 Accession #: P39905 ELISA Kit: RayBio Human GDNF ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	GRO alpha (CXCL1) Gene Symbol: CXCL1 GRO GRO1 GROA MGSA SCYB1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA
GRO alpha/beta/gamma Accession #: P09341/P19875/P19876	HGF Gene Symbol: MET Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 SCYA1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA
IGFBP-1 Gene Symbol: IGFBP1 IBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA	IGFBP-2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 IBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-4 Gene Symbol: IGFBP4 IBP4 Gene ID: 3487 Accession #: P22692 ELISA Kit: RayBio Human IGFBP-4 ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA
IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-2 Gene Symbol: IL2 IL-2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700 ELISA Kit: RayBio Human IL-3 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA
IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA	IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p40/p70 Gene Symbol: IL12A NKSF1 Gene ID: 3592 Accession #: P29459
IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	IL-16 Gene Symbol: IL16 Gene ID: 3603 Accession #: Q14005 ELISA Kit: RayBio Human IL-16 ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	Leptin Gene Symbol: LEP OB OBS Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA
LIF Gene Symbol: LIF HILDA Gene ID: 3976 Accession #: P15018 ELISA Kit: RayBio Human LIF ELISA	LIGHT (TNFSF14) Gene Symbol: TNFSF14 HVEML LIGHT UNQ391/PRO726 Gene ID: 8740 Accession #: O43557 ELISA Kit: RayBio Human LIGHT ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 SCYA2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MCP-2 (CCL8) Gene Symbol: CCL8 MCP2 SCYA10 SCYA8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA	MCP-3 (MARC/CCL7) Gene Symbol: CCL7 MCP3 SCYA6 SCYA7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA
MCP-4 (CCL13) Gene Symbol: CCL13 MCP4 NCC1 SCYA13 Gene ID: 6357 Accession #: Q99616 ELISA Kit: RayBio Human MCP-4 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	MDC (CCL22) Gene Symbol: CCL22 MDC SCYA22 A-152E5.1 Gene ID: 6367 Accession #: O00626 ELISA Kit: RayBio Human MDC ELISA	MIF Gene ID: 4282 Accession #: P14174 ELISA Kit: RayBio Human MIF ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA
MIP-1 beta (CCL4) Gene ID: 388372; 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MIP-1 delta (CCL15) Gene Symbol: CCL15 MIP5 NCC3 SCYA15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 LARC MIP3A SCYA20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA	NAP-2 (PPBP/CXCL7) Gene Symbol: PPBP CTAP3 CXCL7 SCYB7 TGB1 THBGB1 Gene ID: 5473 Accession #: P02775 ELISA Kit: RayBio Human NAP-2 ELISA	NT-3 Gene Symbol: NTF3 Gene ID: 4908 Accession #: P20783 ELISA Kit: RayBio Human NT-3 ELISA
NT-4 Gene Symbol: NTF4 NTF5 Gene ID: 4909 Accession #: P34130 ELISA Kit: RayBio Human NT-4 ELISA	Oncostatin M Gene Symbol: OSM Gene ID: 5008 Accession #: P13725 ELISA Kit: RayBio Human OSM ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 BNSP OPN PSEC0156 Gene ID: 6696 Accession #: P10451 ELISA Kit: RayBio Human OPN ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B OCIF OPG Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	PARC (CCL18) Gene Symbol: CCL18 AMAC1 DCCK1 MIP4 PARC SCYA18 Gene ID: 6362 Accession #: P55774 ELISA Kit: RayBio Human PARC ELISA
PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	PLGF Gene Symbol: PGF PGFL PLGF Gene ID: 5228 Accession #: P49763 ELISA Kit: RayBio Human PLGF ELISA	RANTES (CCL5) Gene Symbol: CCL5 SCYA5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	SCF Gene Symbol: KITLG SCF Gene ID: 4254 Accession #: P21583 ELISA Kit: RayBio Human SCF ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 SDF1 SDF1A SDF1B Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA
TARC (CCL17) Gene Symbol: CCL17 SCYA17 TARC Gene ID: 6361 Accession #: Q92583 ELISA Kit: RayBio Human TARC ELISA	TGF beta 1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	TGF beta 2 Gene Symbol: TGFB2 Gene ID: 7042 Accession #: P61812 ELISA Kit: RayBio Human TGF-beta 2 ELISA	TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600	Thrombopoietin (TPO) Gene Symbol: THPO MGDF Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA
TIMP-1 Gene Symbol: TIMP1 CLGI TIMP Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA TNFB TNFSF1 Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array C5 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Acosta JC, O'Loghlen A, Banito A, Guijarro MV, Augert A, et al. Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence. Cell. 2008;133:1006-1018.
Species: Human
Sample type: Conditioned Media (Fibroblast cell-cultured medium)
McAllister SS, Gifford AM, Greiner AL, Kelleher SP, Saelzer MP, et al. Systemic Endocrine Instigation of Indolent Tumor Growth Requires Osteopontin. Cell. 2008;133:994-1005.
Species: Human
Sample type: Plasma (Plasma from mice with human tumor xenografts)
Kim M, Oskarsson T, Acharyya S, Nguyen D, Zhang X, et al.Tumor Self-Seeding by Circulating Cancer Cells. Cell. 2009;139(7):1315-1326.
Species: Human
Sample type: Conditioned Media (Cell-conditioned media)
Su S., Liu Q., Chen Jin., et al. A Positive Feedback Loop between Mesenchymal-like Cancer Cells and Macrophages Is Essential to Breast Cancer Metastasis. Cancer Cell. 2014 May 12;25(5):605-20. doi: 10.1016/j.ccr.2014.03.021.
Species: Human
Sample type: Conditioned Media (Mesenchymal-like Cancer cells)
Vermeulen L., De Sousa E Melo F., van der Heijden M., et al. Wnt activity defines colon cancer stem cells and is regulated by the microenvironment. Nature Cell Biology 12, 468 - 476 (2010); doi:10.1038/ncb2048
Species: Human
Sample type: Conditioned Media (Myofibroblasts conditioned media)
Acosta, Juan Carlos, et al. "A complex secretory program orchestrated by the inflammasome controls paracrine senescence." Nature cell biology 15.8 (2013): 978.
Species: Human
Sample type: Conditioned Media (senescene model - HEK293T and IMR90 cells)
Pislaru SV, Harbuzariu A, Gulati R, Witt T, et al. Magnetically Targeted Endothelial Cell Localization in Stented Vessels. J Am Coll Cardiol. 2006; 48(9):1839-1845.
Species: Human
Sample type: Conditioned Media (Endothelial cell-conditioned medium)
Trian T., Allard B., Dupin I., et al. House dust mites induce proliferation of severe asthmatic smooth muscle cells via an epithelium-dependent pathway. Am J Respir Crit Care Med. 2015 Mar 1;191(5):538-46. doi: 10.1164/rccm.201409-1582OC
Species: Human
Sample type: Conditioned Media (Bronchial epithelial cells)
Shir, A et al. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. PLoS Med. Volume 3, Issue 1, Pages e6, Jan 2006
Species: Human
Sample type: Conditioned Media (poly IC-transfected cells)
Korkaya H, Kim G, Davis A, et al. Activation of an IL-6 Inflammatory Loop Mediates Trastuzumab Resistance in HER2 Overexpressing Breast Cancers by Expanding the Cancer Stem Cell Population. Molecular cell 2012;47(4):570-584. doi:10.1016/j.molcel.2012.06.014.
Species: Human
Sample type: Conditioned Media (MCF-7 breast carcinoma cell lines)

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I highly recommend this cytokine array kit! It is very easy to use and generates great results. Be sure to set up proper experimental conditions, I used cell culture supernates which yielded much less background than cell lysates.

Regina
University of Texas Medical Branch Galveston 11/04/2022, 12:34 AM

Works best for invitro mamalian cell cytokine assay

Deepak Chouhan
Forsyth Institute 08/09/2022, 06:33 PM

I really liked this kit. It was very easy to use and came with detailed instructions as well troubleshooting tips. I have purchased another similar kit after using this. Highly recommend.

Aditi Kothari
UNC Chapel Hill 07/12/2022, 11:14 AM

The protocol is easy to follow. This kit only needs a small sample volume and can detect a low concentration of cytokine signaling.

Zhang
University of Kansas 04/16/2022, 08:53 PM

The Human Cytokine Array C5 kit gave me satisfied candidates under the conditioned medium. I strongly recommend this!!

Jung
Winship Cancer Institute of Emory University 06/19/2021, 11:08 PM

easy to use and analysis, got excellent results!

Kenneth
NC State University 04/06/2021, 11:01 PM

Simple to use and detects many cytokines

Jose
Johns Hopkins University School of Medicine 03/19/2021, 04:49 PM

It works great using the cell growth media.

DYan
Emory University 01/21/2021, 03:21 AM

I used on mammalian cell culture supernatant it and works well

Vaishnavi
Indiana University 01/07/2021, 02:46 PM

Kit worked well with conditioned media from smooth muscle cells. Provides a lot of data in a small amount of time.

Craig
University of Nevada School of Medicine 03/21/2020, 12:01 PM

I wanted to get something that can work on primary cell line and it turned out good. Results received after experiments had let us plan on which cytokine study we might need to work on for further study. Kit is simple and was of good quality.

Mubeen
University of Texas Health Science Center San Antonio 03/02/2020, 10:32 AM

Easy to use, works well.

Erick
East Tennessee State University 01/17/2020, 04:52 PM

Easy to use, and protocols are easy to follow. Recommended to fellow colleagues.

Raheel
UCSF 12/03/2019, 03:19 AM

Protocol is simple and worked great with our conditioned media!

S Chaudhary
UNMC 07/18/2019, 11:30 PM

Simple and easy protocol with clean results.

Manali
Augusta University 05/03/2019, 10:17 AM

The protocol suggested in the kit works very well for all arrays. Even if you need to optimize the time using the short exposition time suggested in the protocol, your array development will work great as usual!

Thaiz
Augusta University 12/11/2018, 01:34 PM

Worked great with conditioned media. Followed the protocol in the kit and the results were super clean and of excellent quality.

Suchitra
Stanford University 11/13/2018, 04:39 PM

it's an easy kit to use. However, I was not very impressed with their excel software. there was a mistake which we caught and ask them to fix bit they didn't even give us a discount. Other than it's straightforward and give you great results.

Harmony
University of the pacific 09/13/2018, 11:37 AM

Worked great for med-high abundant proteins. Customer service was spectacular

P V raj
UCLA 07/20/2018, 07:56 PM

This kit can detect huge amount of different cytokines and the price is only a little bit more than the kits that only test around 10 cytokines.

Joe wong
Nanjing Medical University 05/09/2018, 05:50 PM

works good with condition media

Himanshu Arora 04/26/2018, 05:16 PM

Human Cytokine Array C5 kit is simple to use, has high quality and results are reproducible. Fast delivery from Raybiotech. But the value for the money is a little high.

Kenneth 04/11/2018, 11:05 AM

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