Cytokine Arrays

Many biological processes such as apoptosis, inflammation, angiogenesis, immune response and migration often accompany changes of cytokine expression levels. Because of the extensive cross-talk between cytokines, a complete analysis of biological responses and functions must be obtained through multiplex assays. Cytokine antibody arrays allow a much broader view of protein activity than can be obtained with single-target ELISAs and Western blots. Moreover, antibody array screening improves the chances for discovering key factors, disease mechanisms or biomarkers related to cytokine signaling.

RayBiotech offers over 80 Cytokine Antibody Arrays, detecting between 10 - 1,000 cytokines in one assay!

Quantibody^® Multiplex ELISA Cytokine Arrays

The Quantibody arrays are quantitative multiplex ELISA arrays featuring fluorescent detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection. Cytokine standards are provided with the array for calculation of target protein concentrations.

All Quantibody arrays feature the sandwich immunoassay principle, utilizing an immobilized capture antibody along with a corresponding biotinylated detection antibody.

Working sample volume: 50-100 µl

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L-Series High-Density Cytokine Arrays

Utilizing direct antigen-labeling technology, RayBiotech has enabled the largest commercially available antibody array to date. With the RayBio® Biotin Label-based Antibody Array (“L-Series”) researchers can obtain a broad, panoramic view of protein expression. Up to 1000 target proteins can be simultaneously detected, including cytokines, chemokines, adipokines, growth factors, proteases, soluble receptors, adhesion molecules, and other proteins, making this array ideally suited for biomarker discovery studies and exploratory screens.

Working sample volume: 20-100 µl

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C-Series Membrane Cytokine Arrays

The C-Series arrays are semi-quantitative, measuring the relative protein expression levels. C-Series features chemiluminescent signal detection. The antibodies are spotted on nitrocellulose membrane solid supports and are handled in a very similar manner to Western blots.

All C-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.

Working sample volume: 1000 µl

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G-Series Glass Slide Cytokine Arrays

The G-Series arrays are semi-quantitative, measuring the relative protein expression levels. G-Series features fluorescent signal detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection.

All G-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.