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ELISA Kits

RayBiotech manufactures over 2,600 ISO certified and rigorously tested ELISA kits, all backed by our 90-day 100% satisfaction guarantee. You can evaluate our kits with no risks and no worries. All RayBio® ELISAs are manufactured in the USA. Learn about our available full ELISA GLP testing services.

 

Sandwich ELISA Detection

Sandwich ELISA

Assay Principle
Sandwich-based (matched antibody pairs)
Targets Detected Cytokines, growth factors, soluble receptors, and other soluble proteins
Sample Types Plasma, serum, cell culture media, lysates, clarified body fluids
Application Protein quantification; Validation of array results; Single target ELISA
Species Human | Mouse | Rat | Cow | Dog | Horse | Cat | Sheep | Pig | Rabbit | Rhesus macaque | Cotton rat

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HSP27 (S82) Phospho-ELISA

Phosphorylation ELISA

Assay Principle
Sandwich-based (matched antibody pairs)
Targets Detected Phosphorylated MAPK, Akt, EGFR, Stat, mTOR, and other signaling molecules
Sample Types Cell & tissue lysates
Application Phospho-protein detection; phosphorylation pathway analysis
Species Human | Mouse | Rat

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Transcription Factor ELISA Detection

Transcription Factor Activity Assay

Assay Principle
Direct (Transcription Factor-DNA Complex)
Targets Detected Active transcription factors
Sample Types Nuclear extracts, whole cell lysates
Application Semi-quantitative detection of transcription factor-DNA complexes in nuclear extracts
Species Human 

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EIA Detection

EIA

Assay Principle
Competition-based
Targets Detected Adipokines, peptide hormones
Sample Types Plasma, serum, cell culture media, lysates, clarified body fluids
Application Protein quantification; Validation of array results; Single target ELISA
Species Multi-species detection (all kits): human, mouse, rat

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Cell-Based ELISA Assay Principle

Cell-Based Phosphorylation ELISA

Assay Principle
Direct
Targets Detected Phosphorylated EGFRs, JNK, ERK, STATs, Phosphotyrosine, and other signaling molecules
Sample Types Adherent cell lines
Application Phospho-protein detection; phosphorylation pathway analysis
Species Human | Mouse | Rat

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ELISA Standard Curve

SpeedELISA

Assay Principle Compressed work flow, Sandwich-based (matched antibody pairs)
Targets Detected Cytokines, growth factors, soluble receptors, and other soluble proteins
Sample Types Plasma, serum, cell culture media*
Application Three hour total processing time; Cytokine quantification; Validation of array results; Single target ELISA
Species Human | Mouse | Rat

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