ELISA Services

With ELISA Services from RayBiotech, you get the confidence of studies done by our experienced scientific team using our proven RayBio® ELISA platform.

We can accelerate your study by using one of our off-the-shelf RayBio® ELISA Kits, which cover 6,000 targets in 17 species, or we can customize an assay to meet your specific project needs.

Every ELISA service project is performed in our GLP-compliant lab using assays which are manufactured in accordance with GMP regulations and an ISO 13485 certified quality management system.

Why You Should Choose Our ELISA Services

For Our Reliable RayBio® ELISA technology

Because we recognize that quality data can only be generated when you use high-quality kits and reagents, we perform all service projects using ELISA kits we manufacture in-house in our facility in Peachtree Corners, Georgia, USA. We’ve found that this is the most reliable way we can ensure reliable data, whether we conduct the study or you do.

For Our Years of Experience

Founded in 2001 as the first company to commercialize the antibody array, we continue to use our decades of experience in multiplex, high-throughput protein analysis to deliver outstanding protein analysis and antibody-related services to the research community.

What you can expect

RayBiotech’s ELISA Service process is streamlined and straightforward:


  1. Finalize your service agreement.
  2. Prepare your samples for shipment—you can find instructions here.
  3. Fill out a Service Form and include it with your shipment.
  4. Ship us your samples.


  1. Once we receive your samples, we’ll send you an email.
  2. We’ll conduct your studies, keeping you informed about any issues or delays
  3. We’ll send back a detailed data analysis in Excel and PDF formats which includes:
    1. Standard curves in log-log format for each analyte.
    2. ODs and averages provided.
    3. Sample concentrations for each target, including original and calculated (accounting for dilutions).

Ready for RayBiotech to start working on your ELISA Service?

Frequently Asked Questions

Still have questions?

On each 96 well plate, an 8-point standard curve is run with duplicate wells. The remaining wells may then be used for samples (duplicates are strongly recommended). If the entire plate is used in 1 experiment, the remaining 80 wells will accommodate 40 samples in duplicate. If not run all at once, a new standard curve must be included with every run, reducing the number of potential samples by 8 (to account for the new standard curve points).

The ELISAs with a longer, typically 4 - 7 week, lead times contain antibody pairs and standards that have been validated for multiplex array products, but not for the 96-well plate ELISA format. As a result, they require a development phase that RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests and will, thereafter, have a typical 1 - 2 day lead time for orders placed.

Our sandwich ELISA kits are evaluated for intra-assay reproducibility and precision by running 2-3 positive control samples in duplicate on a single plate. Inter-assay reproducibility is evaluated by at least 2 independent experiments with 2-3 positive control samples and a full standard curve. Lot-to-lot consistency is tested by comparing calculated concentrations of 2-3 positive control samples with calibration curves of current lot and previous lot.

Recovery is determined by spiking various levels of target protein into biological samples. Dilution linearity is tested by performing 2-fold and 4-fold dilutions of biological samples. Recoveries typically range from 80-130%.

The ELISA kits with catalog numbers ending in “-CL” are optimized and validated for use with cell and tissue lysate samples. All other ELISA and EIA kits (catalog numbers without “-CL”) have not been validated by RayBiotech with cell or tissue lysates, however RayBio kits can generally accommodate many different samples types. All RayBio Phosphorylation ELISA kits are validated with cell lysates.

Still have questions?

Featured Resources

Get additional information to help you advance your studies from articles on the science underlying antibody-based techniques to video protocols, application notes, and more.

    Learning Center
    ELISA Vs. Immuno-PCR Vs. Simoa: Comparison Of Protein Detection Tools.
    Technical Information
    High-Throughput Cytokine Quantification Using Multiplex ELISA Microarrays
    Rapid ELISA-Based Measurement of Protein Phosphorylation Using RayBio® Phosphorylation ELISA Kits

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