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Home/Search results/Products/Arrays/Antibody Arrays/G-Series: Glass Slide-Based Arrays/Acute Kidney Injury Arrays/Human AKI Array G1

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Antigen Information

Gene Symbols:

ALB | B2M | CALB1 | CCL2 | CLU | CST3 | CXCL10 | CXCL16 | FABP1 | GPNMB View more

Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

4 or 8 Sample Kit

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
TIM-1 (KIM-1) Gene Symbol: HAVCR1 Gene ID: 26762 Accession #: Q96D42 ELISA Kit: RayBio Human TIM-1 ELISA	Albumin Gene Symbol: ALB Gene ID: 213 Accession #: P02768 ELISA Kit: RayBio Human Albumin ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Gene ID: 6696 Accession #: P10451 ELISA Kit: RayBio Human OPN ELISA	TFF3 (Trefoil Factor 3) Gene Symbol: TFF3 Gene ID: 7033 Accession #: Q07654 ELISA Kit: RayBio Human Trefoil Factor 3 ELISA	Beta-2 Microglobulin Gene Symbol: B2M Gene ID: 567 Accession #: P61769 ELISA Kit: RayBio Human B2M ELISA
Clusterin Gene Symbol: CLU Gene ID: 1191 Accession #: P10909 ELISA Kit: RayBio Human Clusterin ELISA	CXCL16 Gene Symbol: CXCL16 Gene ID: 58191 Accession #: Q9H2A7 ELISA Kit: RayBio Human CXCL16 ELISA	Osteoactivin (GPNMB) Gene Symbol: GPNMB Gene ID: 10457 Accession #: Q14956 ELISA Kit: RayBio Human Osteoactivin ELISA	FABP1 Gene Symbol: FABP1 Gene ID: 2168 Accession #: P07148	MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA
TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA	Calbindin Gene Symbol: CALB1 Gene ID: 793 Accession #: P05937 ELISA Kit: RayBio Human Calbindin ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	Cystatin C Gene Symbol: CST3 Gene ID: 1471 Accession #: P01034 ELISA Kit: RayBio Human Cystatin C ELISA	HGF Gene Symbol: HGF Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA
MIF Gene Symbol: MIF Gene ID: 4282 Accession #: P14174 ELISA Kit: RayBio Human MIF ELISA	Lipocalin-2 (NGAL) Gene Symbol: LCN2 Gene ID: 3934 Accession #: P80188 ELISA Kit: RayBio Human Lipocalin-2 ELISA	TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	VCAM-1 (CD106) Gene Symbol: VCAM1 Gene ID: 7412 Accession #: P19320 ELISA Kit: RayBio Human VCAM-1 ELISA	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity

Kit Components

Human Acute Kidney Injury Antibody Array G1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Manual

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