Human Angiogenesis Array G1

RayBio® G-Series Human Angiogenesis Antibody Array 1 Kit. Detects 20 Human Angiogenic Factors. Suitable for all liquid sample types.

AAH-ANG-G1-4
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Antigen Information

Gene Symbols:
ANG | CCL2 | CCL5 | CXCL1 | CXCL2 | CXCL3 | CXCL5 | CXCL8 | EGF | FGF2   View more

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Angiogenin
EGF
ENA-78
(CXCL5)
bFGF
GRO alpha/beta/gamma
IFN-gamma
IGF-1
IL-6
IL-8
(CXCL8)
Leptin
MCP-1
(CCL2)
PDGF-BB
PLGF
RANTES
(CCL5)
TGF beta 1
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
VEGF-A
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Angiogenesis Antibody Array G1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Shomer E., Katzenell S., Zipori Y., et al. Microvesicles of pregnant women receiving low molecular weight heparin improve trophoblast function. Thromb Res. 2016 Jan;137:141-7. doi: 10.1016/j.thromres.2015.11.026. Epub 2015 Nov 23.
    Species: Human
    Sample type: Conditioned Media
  2. Barcena C., Stefanociv M., Tutusaus A., et al. Angiogenin secretion from hepatoma cells activates hepatic stellate cells to amplify a self-sustained cycle promoting liver cancer. Sci Rep. 2015 Jan 21;5:7916. doi: 10.1038/srep07916.
    Species: Human
    Sample type: Conditioned Media
  3. Iman V., Karimian H., Mohan S., et al. In vitro and in vivo anti-angiogenic activity of girinimbine isolated from Murraya koenigii. Drug Des Devel Ther. 2015; 9: 1281-1292. doi: 10.2147/DDDT.S71557
    Species: Human
    Sample type: Conditioned Media
  4. Jose S., Hughbanks ML., Binder BY., Ingvale GC., Leach JK. Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels. Acta Biomater. 2014 May;10(5):1955-64. doi: 10.1016/j.actbio.2014.01.020
    Species: Human
    Sample type: Hydrogel
  5. Tura, Olga, et al. "Late outgrowth endothelial cells resemble mature endothelial cells and are not derived from bone marrow." Stem Cells 31.2 (2013): 338-348.
    Species: Human
    Sample type: Conditioned Media
  6. Roy, Rajika, et al. "Cardioprotection by placenta-derived stromal cells in a murine myocardial infarction model." Journal of Surgical Research 185.1 (2013): 70-83.
    Species: Human
    Sample type: Conditioned Media
  7. van Mil A., Grundmann S., Goumans MJ., et al. MicroRNA-214 inhibits angiogenesis by targeting Quaking and reducing angiogenic growth factor release. Cardiovasc Res. 2012 Mar 15;93(4):655-65. doi: 10.1093/cvr/cvs003.
    Species: Human
    Sample type: Conditioned Media
  8. Hoch A., Binder B., Genetos D., Leach J. Differentiation-dependent secretion of proangiogenic factors by mesenchymal stem cells. PLoS One. 2012;7(4):e35579. doi: 10.1371/journal.pone.0035579.
    Species: Human
    Sample type: Conditioned Media
  9. Sugano, G., et al. "Milk fat globule—epidermal growth factor—factor VIII (MFGE8)/lactadherin promotes bladder tumor development." Oncogene 30.6 (2011): 642-653.
    Species: Mouse
    Sample type: Tissue lysate
Works for my spleen lysate supernatant.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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