Human Apoptosis Array G1

RayBio® G-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$540.35  
AAH-APO-G1-4
+

Antigen Information

Gene Symbols:
BAD | BAX | BCL2 | BCL2L11 | BCL2L2 | BID | BIRC3 | BIRC5 | BIRC7 | CASP3   View more

Assay Format

Number of Targets Detected:
43
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
bad
bax
  • Gene Symbol: BAX
  • Gene ID: 581
  • Accession #: Q07812
bcl-2
  • Gene Symbol: BCL2
  • Gene ID: 596
  • Accession #: P10415
bcl-w
  • Gene Symbol: BCL2L2
  • Gene ID: 599
  • Accession #: Q92843
BID
BIM
  • Gene Symbol: BCL2L11
  • Gene ID: 10018
  • Accession #: Q43521
Caspase-3
  • Gene Symbol: CASP3
  • Gene ID: 836
  • Accession #: P42574
Caspase-8
  • Gene Symbol: CASP8
  • Gene ID: 841
  • Accession #: Q14790
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
cIAP-2
Cytochrome C
DR6
(TNFRSF21)
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
HSP27
HSP60
  • Gene Symbol: HSPA14
  • Gene ID: 51182
  • Accession #: Q0VDF9
HSP70
HTRA2
IGF-1
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-5
IGFBP-6
IGF-1 R
livin
p21
  • Gene Symbol: CDKN1A
  • Gene ID: 1026
  • Accession #: P38936
p27
p53
SMAC
Survivin
(BIRC5)
  • Gene Symbol: BIRC5
  • Gene ID: 332
  • Accession #: O15392
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R1
(TNFRSF10A/DR4)
TRAIL R2
(TNFRSF10B/DR5)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
XIAP

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Apoptosis Antibody Array G1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Qin Y., Zhang S., Den S., et al. Epigenetic silencing of miR-137 induces drug resistance and chromosomal instability by targeting AURKA in multiple myeloma. Leukemia. 2017 Jan 6. doi: 10.1038/leu.2016.325.
    Species: Human
    Sample type: Cell Lysate (H929 and mir-137 overexpressing cells)
  2. The Presence of HIV-1 Tat Second Exon Delays Fas-Mediated Apoptosis in CD4+ T lymphocytes: a Potential Mechanism for Persistent Viral Production
    Species: Human
    Sample type: Cell Lysate (Jurkat cell variants)
  3. López-Huertas, María Rosa, et al. "The Presence of HIV-1 Tat Protein Second Exon Delays Fas Protein-mediated Apoptosis in CD4+ T Lymphocytes A POTENTIAL MECHANISM FOR PERSISTENT VIRAL PRODUCTION." Journal of Biological Chemistry 288.11 (2013): 7626-7644.
    Species: Human
    Sample type: Cell Lysate (Jurkat-Tat101 and Jurkat-Tat72 cells)
  4. Qin M., Luo Y., Meng X., et al. Myricitrin attenuates endothelial cell apoptosis to prevent atherosclerosis: An insight into PI3K/Akt activation and STAT3 signaling pathways. Vascul Pharmacol. 2015 Jul;70:23-34. doi: 10.1016/j.vph.2015.03.002
    Species: Human
    Sample type: Cell Lysate (HUVEC Cells)
  5. Asif M., Shafaei A., Jafari S., et al. Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study. Toxicol Lett. 2016 Aug 22;257:84-96. doi: 10.1016/j.toxlet.2016.05.027.
    Species: Human
    Sample type: Cell Lysate (HCT116 colorectal carcinoma cells)
  6. Ibrahim M., et al. ?-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-?B and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther. 2014 Sep 27;8:1629-47. doi: 10.2147/DDDT.S66105
    Species: Human
    Sample type: Cell Lysate (Breast cancer cells, MCF-10 and MCF-7)
  7. Li X., Liu X., Xu Y., et al. Expression profile of apoptotic and proliferative proteins in hypoxic HUVEC treated with statins. Int J Oncol. 2015 Feb;46(2):677-84. doi: 10.3892/ijo.2014.2780.
    Species: Human
    Sample type: Cell Lysate (HUVEC Cells)
  8. Chen, Yao, et al. "WAP four-disulfide core domain protein 2 gene (WFDC2) is a target of estrogen in ovarian cancer cells." Journal of ovarian research 9.1 (2016): 10.
    Species: Human
    Sample type: Cell Lysate ( HO8910-NA and HO8910-209 cells)
  9. Sidhu P., et al. Anticancer activity of VDR-coregulator inhibitor PS121912. Cancer Chemotherapy and Pharmacology Published Online August 2014. DOI 10.1007/s00280-014-2549-y
    Species: Human
    Sample type: Cell Lysate (HL-60 cells)
  10. Mohidin T., Ng C. BARF1 gene silencing triggers caspase-dependent mitochondrial apoptosis in Epstein-Barr virus-positive malignant cells. J Biosci. 2015 Mar;40(1):41-51.
    Species: Human
    Sample type: Cell Lysate (AG876 and Hone-Akata cells transfected with BARF1 siRNA)
A great assay for a fairly wide array of apoptosis proteins and if you lack the proper scanners the company will quickly scan and analyze your samples at no cost.

Columbia University
A very high-throughput, sensitive product to detect the apoptosis.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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