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Home/Search results/Products/Arrays/Antibody Arrays/G-Series/Apoptosis Arrays/Human Apoptosis Array G1

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Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

4 or 8 Sample Kit

Protein Information

Gene Symbols:

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
bad Gene Symbol: bad Gene ID: 572 Accession #: Q92934 ELISA Kit: RayBio Human BAD ELISA	bax Gene Symbol: BAX Gene ID: 581 Accession #: Q07812	bcl-2 Gene Symbol: BCL2 Gene ID: 596 Accession #: P10415	bcl-w Gene Symbol: BCL2L2 Gene ID: 599 Accession #: Q92843	BID Gene Symbol: BID Gene ID: 637 Accession #: P55957 ELISA Kit: RayBio Human BID ELISA
BIM Gene Symbol: BCL2L11 Gene ID: 10018 Accession #: Q43521	Caspase-3 Gene Symbol: CASP3 Gene ID: 836 Accession #: P42574	Caspase-8 Gene Symbol: CASP8 Gene ID: 841 Accession #: Q14790	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA
cIAP-2 Gene Symbol: BIRC3 Gene ID: 330 Accession #: Q13489 ELISA Kit: RayBio Human cIAP2 ELISA	Cytochrome C	DR6 (TNFRSF21) Gene Symbol: TNFRSF21 Gene ID: 27242 Accession #: O75509 ELISA Kit: RayBio Human DR6 ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Gene ID: 356 Accession #: P48023 ELISA Kit: RayBio Human FasL ELISA
HSP27 Gene Symbol: HSPB1 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human HSP27 ELISA	HSP60 Gene Symbol: HSPA14 Gene ID: 51182 Accession #: Q0VDF9	HSP70 Gene Symbol: HSPA4 Gene ID: 3308 Accession #: P34932 ELISA Kit: RayBio Human HSP70 ELISA	HTRA2 Gene Symbol: HTRA2 Gene ID: 27429 Accession #: O43464 ELISA Kit: RayBio Human HTRA2 ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA
IGF-2 Gene Symbol: Igf2 Gene ID: 3481 Accession #: P01344	IGFBP-1 Gene Symbol: IGFBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-4 Gene Symbol: IGFBP4 Gene ID: 3487 Accession #: P22692 ELISA Kit: RayBio Human IGFBP-4 ELISA
IGFBP-5 Gene Symbol: IGFBP5 Gene ID: 3488 Accession #: P24593 ELISA Kit: RayBio Human IGFBP-5 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Gene ID: 3489 Accession #: P24592 ELISA Kit: RayBio Human IGFBP-6 ELISA	IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069 ELISA Kit: RayBio Human IGF-1 R ELISA	livin Gene Symbol: BIRC7 Gene ID: 79444 Accession #: Q96CA5 ELISA Kit: RayBio Human Livin ELISA	p21 Gene Symbol: CDKN1A Gene ID: 1026 Accession #: P38936
p27 Gene Symbol: SSSCA1 Gene ID: 10534 Accession #: O60232 ELISA Kit: RayBio Human p27 ELISA	p53 Gene Symbol: TP53 Gene ID: 7157 Accession #: P04637 ELISA Kit: RayBio Human p53 ELISA	SMAC Gene Symbol: DIABLO Gene ID: 56616 Accession #: Q9NR28 ELISA Kit: RayBio Human SMAC ELISA	Survivin (BIRC5) Gene Symbol: BIRC5 Gene ID: 332 Accession #: O15392	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA
TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TRAIL R1 (TNFRSF10A/DR4) Gene Symbol: TNFRSF10A Gene ID: 8797 Accession #: O00220 ELISA Kit: RayBio Human TRAIL R1 ELISA	TRAIL R2 (TNFRSF10B/DR5) Gene Symbol: TNFRSF10B Gene ID: 8795 Accession #: O14763 ELISA Kit: RayBio Human TRAIL R2 ELISA
TRAIL R3 (TNFRSF10C) Gene Symbol: TNFRSF10C Gene ID: 8794 Accession #: O14798 ELISA Kit: RayBio Human TRAIL R3 ELISA	TRAIL R4 (TNFRSF10D) Gene Symbol: TNFRSF10D Gene ID: 8793 Accession #: Q9UBN6 ELISA Kit: RayBio Human TRAIL R4 ELISA	XIAP Gene Symbol: XIAP Gene ID: 331 Accession #: P98170 ELISA Kit: RayBio Human XIAP ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Apoptosis Antibody Array G1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Protease Inhibitor Cocktail
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Zhao, Jiao, et al. "The influence of simulated microgravity on proliferation and apoptosis in U251 glioma cells." In Vitro Cellular & Developmental Biology-Animal 53.8 (2017): 744-751.
Species: Human
Sample type: Cell Lysate
Asif M., Al-Masoub M., Sultan Kahn M., et al. Molecular mechanisms responsible for programmed cell death-inducing attributes of terpenes from Mesua ferrea stem bark towards human colorectal carcinoma HCT 116 cells. Journal of Applied Biomedicine, Oct 18 2016, epub ahead of print
Species: Human
Sample type: Cell Lysate
Asif M., Shafaei A., Jafari S., et al. Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study. Toxicol Lett. 2016 Aug 22;257:84-96. doi: 10.1016/j.toxlet.2016.05.027.
Species: Human
Sample type: Cell Lysate
Qin Y., Zhang S., Den S., et al. Epigenetic silencing of miR-137 induces drug resistance and chromosomal instability by targeting AURKA in multiple myeloma. Leukemia. 2017 Jan 6. doi: 10.1038/leu.2016.325.
Species: Human
Sample type: Cell Lysate
Chen, Yao, et al. "WAP four-disulfide core domain protein 2 gene (WFDC2) is a target of estrogen in ovarian cancer cells." Journal of ovarian research 9.1 (2016): 10.
Species: Human
Sample type: Cell Lysate
Mohidin T., Ng C. BARF1 gene silencing triggers caspase-dependent mitochondrial apoptosis in Epstein-Barr virus-positive malignant cells. J Biosci. 2015 Mar;40(1):41-51.
Species: Human
Sample type: Cell Lysate
Qin M., Luo Y., Meng X., et al. Myricitrin attenuates endothelial cell apoptosis to prevent atherosclerosis: An insight into PI3K/Akt activation and STAT3 signaling pathways. Vascul Pharmacol. 2015 Jul;70:23-34. doi: 10.1016/j.vph.2015.03.002
Species: Human
Sample type: Cell Lysate
Ibrahim M., et al. ?-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-?B and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther. 2014 Sep 27;8:1629-47. doi: 10.2147/DDDT.S66105
Species: Human
Sample type: Cell Lysate
Li X., Liu X., Xu Y., et al. Expression profile of apoptotic and proliferative proteins in hypoxic HUVEC treated with statins. Int J Oncol. 2015 Feb;46(2):677-84. doi: 10.3892/ijo.2014.2780.
Species: Human
Sample type: Cell Lysate
Sidhu P., et al. Anticancer activity of VDR-coregulator inhibitor PS121912. Cancer Chemotherapy and Pharmacology Published Online August 2014. DOI 10.1007/s00280-014-2549-y
Species: Human
Sample type: Cell Lysate

1 2

Monitored protein expression in HMCB cells. Company’s free scanning of the array allowed us to use this tool and provide quality research experiences for undergraduates.

Julie Maxson
Hiram College 12/03/2021, 08:43 AM

A great assay for a fairly wide array of apoptosis proteins and if you lack the proper scanners the company will quickly scan and analyze your samples at no cost.

Alan Chramiec
Columbia University 11/21/2019, 11:54 PM

A very high-throughput, sensitive product to detect the apoptosis.

Williams Deng 05/02/2018, 05:39 PM

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