Human Growth Factor Array G1

RayBio® G-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.

AAH-GF-G1-4
+

Antigen Information

Gene Symbols:
AREG | CSF1 | CSF1R | CSF2 | CSF3 | EGF | EGFR | FGF2 | FGF4 | FGF6   View more

Assay Format

Number of Targets Detected:
41
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Amphiregulin
bFGF
beta-NGF
EGF
EGFR
FGF-4
FGF-6
FGF-7
(KGF)
GCSF
GDNF
GM-CSF
HB-EGF
HGF
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
M-CSF
M-CSF R
NT-3
NT-4
PDGF R alpha
PDGF R beta
PDGF-AA
PDGF-AB
PDGF-BB
PLGF
SCF
SCF R
(CD117/c-kit)
TGF alpha
TGF beta 1
TGF beta 2
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
VEGF-A
VEGFR2
VEGFR3
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Growth Factor Antibody Array G1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Peloso A., Petrosyan A., Da Sacco S., et al. Renal Extracellular Matrix Scaffolds From Discarded Kidneys Maintain Glomerular Morphometry and Vascular Resilience and Retains Critical Growth Factors. Transplantation. 2015 Sep;99(9):1807-16. doi: 10.1097/TP.0000000000000811.
    Species: Human
    Sample type: Tissue Lysate
  2. Liu D., et al. SIX1 Promotes Tumor Lymphangiogenesis by Coordinating TGF? Signals That Increase Expression of VEGF-C . Cancer Res; Published OnlineFirst August 20, 2014; doi:10.1158/0008-5472.CAN-13-3598
    Species: Human
    Sample type: Conditioned Media
  3. Zhang M., Xu M., Zhou Z., Zhou J., Zhao Y., Wang Z., Lu M. The Differentiation of Human Adipose-Derived Stem Cells towards a Urothelium-Like Phenotype In Vitro and the Dynamic Temporal Changes of Related Cytokines by Both Paracrine and Autocrine Signal Regulation. PLOS One, Published: April 21, 2014, DOI: 10.1371/journal.pone.0095583 [Epub ahead of print]
    Species: Human
    Sample type: Conditioned Media
  4. Jeong, Yun-Mi, et al. "Ultraviolet B preconditioning enhances the hair growth-promoting effects of adipose-derived stem cells via generation of reactive oxygen species." Stem cells and development 22.1 (2012): 158-168.
    Species: Human
    Sample type: Cell Lysate
  5. Balasubramanian, Sudha, et al. "Higher propensity of Wharton's jelly derived mesenchymal stromal cells towards neuronal lineage in comparison to those derived from adipose and bone marrow." Cell Biology International 37.5 (2013): 507-515.
    Species: Human
    Sample type: Conditioned Media
  6. Balasubramanian S., Venugopal P., Sundarraj S., et al. Comparison of chemokine and receptor gene expression between Wharton's jelly and bone marrow-derived mesenchymal stromal cells. Cytotherapy. 2012 Jan;14(1):26-33. doi: 10.3109/14653249.2011.605119.
    Species: Human
    Sample type: Conditioned Media
  7. Petschnik, Anna E., et al. "A novel xenogeneic co-culture system to examine neuronal differentiation capability of various adult human stem cells." PloS one 6.9 (2011): e24944.
    Species: Rat
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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