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Home/Search results/Products/Arrays/Antibody Arrays/G-Series/Obesity Arrays/Human Obesity Array G1

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Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

4 or 8 Sample Kit

Protein Information

Gene Symbols:

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 Gene ID: 3604 Accession #: Q07011 ELISA Kit: RayBio Human TNFRSF9 ELISA	ACE-2 Gene Symbol: ACE2 Gene ID: 59272 Accession #: Q9BYF1	Adiponectin (ACRP30) Gene Symbol: ADIPOQ Gene ID: 9370 Accession #: Q15848 ELISA Kit: RayBio Human Acrp30 ELISA	Adipsin (Complement Factor D) Gene Symbol: CFD Gene ID: 1675 Accession #: P00746 ELISA Kit: RayBio Human Adipsin ELISA	AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA
Angiopoietin-1 Gene Symbol: ANGPT1 Gene ID: 284 Accession #: Q15389 ELISA Kit: RayBio Human ANGPT1 ELISA	Angiopoietin-2 Gene Symbol: ANGPT2 Gene ID: 285 Accession #: O15123 ELISA Kit: RayBio Human ANGPT2 ELISA	ANGPTL4 Gene Symbol: ANGPTL4 Gene ID: 51129 Accession #: Q9BY76 ELISA Kit: RayBio Human ANGPTL4 ELISA	CRP (C-Reactive Protein) Gene Symbol: CRP Gene ID: 1401 Accession #: P02741 ELISA Kit: RayBio Human CRP ELISA	ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA
Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA	FGF-6 Gene Symbol: FGF6 Gene ID: 2251 Accession #: P10767 ELISA Kit: RayBio Human FGF-6 ELISA	Growth Hormone Gene Symbol: GH1 Gene ID: 2688 Accession #: P01241 ELISA Kit: RayBio Human GH ELISA	HCC-4 (CCL16) Gene Symbol: CCL16 Gene ID: 6360 Accession #: O15467 ELISA Kit: RayBio Human HCC-4 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA
IGFBP-1 Gene Symbol: IGFBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA	IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069 ELISA Kit: RayBio Human IGF-1 R ELISA
IL-1 R4 (ST2) Gene Symbol: IL1RL1 Gene ID: 9173 Accession #: Q01638 ELISA Kit: RayBio Human IL-1 R4 ELISA	IL-1 R1 Gene Symbol: IL1R1 Gene ID: 3554 Accession #: P14778 ELISA Kit: RayBio Human IL-1 sRI ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-11 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809 ELISA Kit: RayBio Human IL-11 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA
IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-6 R Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA
Insulin Gene Symbol: INS Gene ID: 3630 Accession #: P01308 ELISA Kit: RayBio Human Insulin ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	Leptin R Gene Symbol: LEPR Gene ID: 3953 Accession #: P48357	Leptin Gene Symbol: LEP Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA	LIF Gene Symbol: LIF Gene ID: 3976 Accession #: P15018 ELISA Kit: RayBio Human LIF ELISA
Lymphotactin (XCL1) Gene Symbol: XCL1 Gene ID: 6375 Accession #: P47992 ELISA Kit: RayBio Human Lympotactin ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MCP-3 (MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	MIF Gene Symbol: MIF Gene ID: 4282 Accession #: P14174 ELISA Kit: RayBio Human MIF ELISA
MIP-1 beta (CCL4) Gene Symbol: CCL4 Gene ID: 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MSP alpha/beta Gene Symbol: MST1 Gene ID: 4485 Accession #: P26927 ELISA Kit: RayBio Human MSP ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	Oncostatin M Gene Symbol: OSM Gene ID: 5008 Accession #: P13725 ELISA Kit: RayBio Human OSM ELISA	PAI-1 Gene Symbol: SERPINE1 Gene ID: 5054 Accession #: P05121 ELISA Kit: RayBio Human PAI-1 ELISA
PARC (CCL18) Gene Symbol: CCL18 Gene ID: 6362 Accession #: P55774 ELISA Kit: RayBio Human PARC ELISA	PDGF-AA Gene Symbol: PDGFA Gene ID: 5154 Accession #: P04085 ELISA Kit: RayBio Human PDGF-AA ELISA	PDGF-AB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-AB ELISA	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	RANTES (CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA
Resistin Gene Symbol: RETN Gene ID: 56729 Accession #: Q9HD89 ELISA Kit: RayBio Human Resistin ELISA	SAA (Serum Amyloid A) Gene Symbol: SAA1 Gene ID: 6288 Accession #: P0DJI8 ELISA Kit: RayBio Human SAA ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA
TECK (CCL25) Gene Symbol: CCL25 Gene ID: 6370 Accession #: O15444 ELISA Kit: RayBio Human TECK ELISA	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA
VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA	XEDAR Gene Symbol: EDA2R Gene ID: 60401 Accession #: Q9HAV5

View Array Map

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Obesity Antibody Array G1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.