Rat Cytokine Array G2

RayBio® G-Series Rat Cytokine Antibody Array 2 Kit. Detects 34 Rat Cytokines. Suitable for all liquid sample types.

AAR-CYT-G2-4
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Antigen Information

Gene Symbols:
AGER | AGRN | CCL2 | CCL20 | CD86 | CNTF | CSF2 | CX3CL1 | CXCL1 | CXCL2   View more

Assay Format

Number of Targets Detected:
34
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Activin A
Agrin
CD86
(B7-2)
beta-NGF
CINC-1
(CXCL1)
CINC-2
CINC-3
CNTF
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GM-CSF
ICAM-1
(CD54)
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 R6
(IL-1 Rrp2)
IL-2
IL-4
IL-6
IL-10
IL-13
Leptin
LIX
L-Selectin
(CD62L)
MCP-1
(CCL2)
MIP-3 alpha
(CCL20)
MMP-8
PDGF-AA
Prolactin R
RAGE
TCK-1
(CXCL7)
TIMP-1
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Rat Cytokine Antibody Array G2 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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