G-Series: Glass Slide Antibody Arrays

Semi-Quantitative, Sandwich-based, Glass Slide Antibody Arrays

Antibody arrays allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid.

RayBio^® Glass Slide-based Antibody Arrays (G-Series) were introduced as a “second generation” array platform after the chemiluminescent membrane arrays (C-Series). The G-Series uses a 75mm x 25mm glass chip platform which delivers easier handling along with the use of much lower sample volumes. The glass slides also utilize a fluorescent signal readout, allowing a wider dynamic detection range than can be achieved by chemiluminescence.

G-Series Antibody Arrays are particularly well-suited for projects with very limited sample volumes (as little as 10 μl per array) and large numbers of samples. Dozens of arrays can be processed per day by a single technician. Furthermore, the slide chamber assembly is compatible with many automated array processing systems, allowing high-throughput studies to be performed with very little hands-on time.

For a comparison & overview of all our antibody array systems, click here.

Compare G-Series Protein Lists Customize Your Array Browse G-Series Arrays

Features

High sensitivity (pg/mL to ng/mL levels)
Large dynamic range of detection (4 orders of magnitude)
Compatible with many sample types
High throughput
Low sample consumption: as little as 10 µl of original sample required per array
Customizable – create a custom array from our list of targets

Research Applications

High-throughput profiling of cytokine expression
Identifying potential molecular targets for drug development
Identifying the molecular mechanisms of drug action<
Identifying crucial factors involved in disease processes
Discovering biomarkers for disease management
Discovering expression patterns for molecular classification of diseases

How It Works

Similar to the C-Series (membrane-based) arrays, G-Series Antibody Arrays utilize the sandwich-ELISA design principle. In this assay, capture antibodies are printed in multiple identical arrays on a standard-sized histology slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are visualized using a fluorescence laser scanner.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech now offers FREE scanning and data extraction service for all G-Series Arrays. Click to learn more

Contents of kit

RayBio^® Cytokine Antibody Array glass slide (4 or 8 arrays per slide)
Biotinylated Detection Antibodies
Streptavidin-Conjugated Fluor
Blocking Buffer
20X Wash Buffer I
20X Wash Buffer II
2X Cell Lysis Buffer
G-Series Antibody Array accessories*
Manual

*Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

Comparison to ELISA

More Data, Less Sample: G-Series Antibody arrays provide high-content screening using less sample volume than ELISA. Only 50 µl needed (after dilution) to detect up to 40 different biomarker proteins. As little as 10 µl original sample!
Global View of Cytokine Expression: Antibody array screening improves the chances for discovering key factors, disease mechanisms or biomarkers related to cytokine signaling.
Greater Sensitivity: As little as 4 pg/ml of MCP-1 can be detected using the G-Series array format. In contrast, our similar MCP-1 ELISA assay has a sensitivity of 40 pg/ml of MCP-1.
Increased Range of Detection: ELISA assays typically detect a concentration range of 100- to 1000-fold, however, RayBiotech arrays can detect IL-2 at concentrations of 25 to 250,000 pg/ml, a range of 10,000-fold.
Better Precision: As determined by densitometry, the inter-array Coefficient of Variation (CV) of spot signal intensities is 5-10%, comparing favorably with ELISA testing (CV = 10-15%).

Glass Slide Microarray Technique

References

Pasquier J., Gosset M., Geyl C., et al. CCL2/CCL5 secreted by the stroma induce IL-6/PYK2 dependent chemoresistance in ovarian cancer. https://www.ncbi.nlm.nih.gov/pubmed/29455640 [view publication]
Product: Human Cytokine Array G1000
Jung YJ, Kim HK, Cho Y, Choi JS, Woo CH, Lee KS, Sul JH, Lee CM, Han J, Park JH, Jo DG, Cho YW. Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes. Sci Adv. 2020 Mar 25;6(13):eaay6721. doi: 10.1126/sciadv.aay6721. PMID: 32232152; PMCID: PMC7096171. [view publication]
Product: Human Obesity Array G1
Wu T., Chang S., Li C., et al. Severe Hepatitis Promotes Hepatocellular Carcinoma Recurrence via NF-κB Pathway-Mediated Epithelial-Mesenchymal Transition after Resection. Clin Cancer Res. 2016 Apr 1;22(7):1800-12. doi: 10.1158/1078-0432.CCR-15-0780. [view publication]
Product: Human Inflammation Array G3
Shi D., Zhang J., Zhou Q., et al. Quantitative evaluation of human bone mesenchymal stem cells rescuing fulminant hepatic failure in pigs. Gut. 2016 Feb 16. pii: gutjnl-2015-311146. doi: 10.1136/gutjnl-2015-311146 [view publication]
Product: Human Cytokine Array G4000
Wang, Z., Niu, Y., Tian, X., Yu, N., Yin, X., Xing, Z., … Wang, C. (2020). Switching On and Off Macrophages by a “Bridge‐Burning” Coating Improves Bone‐Implant Integration under Osteoporosis. Advanced Functional Materials, 2007408. doi:10.1002/adfm.202007408 [view publication]
Product: Rat Cytokine Array GS67
Qin Y., Zhang S., Den S., et al. Epigenetic silencing of miR-137 induces drug resistance and chromosomal instability by targeting AURKA in multiple myeloma. Leukemia. 2017 Jan 6. doi: 10.1038/leu.2016.325. [view publication]
Product: Human Apoptosis Array G1
Buchmann GK, Schürmann C, Warwick T, Schulz MH, Spaeth M, Müller OJ, Schröder K, Jo H, Weissmann N, Brandes RP. Deletion of NoxO1 limits atherosclerosis development in female mice. Redox Biol. 2020 Oct;37:101713. doi: 10.1016/j.redox.2020.101713. Epub 2020 Sep 4. PMID: 32949971; PMCID: PMC7502371. [view publication]
Product: Mouse Cytokine Array G3
Cao B, Zhang N, Zhang Y, Fu Y, Zhao D. Plasma cytokines for predicting diabetic retinopathy among type 2 diabetic patients via machine learning algorithms. Aging (Albany NY). 2020 Dec 11;12. doi: 10.18632/aging.202168. Epub ahead of print. PMID: 33323553. [view publication]
Product: Human Angiogenesis Array GS1 | Human Angiogenesis Array GS2
Raghunatha, P., Vosoughi, A., Kauppinen, T. M., & Jackson, M. F. (2020). Microglial NMDA receptors drive pro‐inflammatory responses via PARP‐1/TRMP2 signaling. Glia. doi:10.1002/glia.23790 [view publication]
Product: Mouse Inflammation Array G1
Ding, Chenyue, et al. "Different therapeutic effects of cells derived from human amniotic membrane on premature ovarian aging depend on distinct cellular biological characteristics." Stem cell research & therapy 8.1 (2017): 173. [view publication]
Product: Human Cytokine Array G2000