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Manual / SDS

View manual for glycan names/descriptions

Description
Citations
Reviews
Files

Assay Format

Number of Targets Detected:

100

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Purified Proteins
Serum
Tissue Lysates

Design Principle:

Label-based
Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

4, 8, or 16 Sample Kit

Introduction

Glycocalyx, literally meaning ‘sugar coat’, is an extracellular polymeric coating surrounding many prokaryotic and eukaryotic cells consisting of glycoproteins, glycolipids, proteoglycans and glycosaminoglycans. The constituents of the glycocalyx play an important role in the process of cell signaling, virus infections, and immunity. However, detection tools for the research of glycobiology are currently in very limited supply.

Raybiotech has pioneered the development of antibody arrays which are now widely applied in the research community with thousands of peer reviewed publications, including Cell and Nature. Taking advantage of advancements in microarray technology developed for antibody arrays, we have developed the first commercially available glycan array for screening protein-carbohydrate interactions. This array will help researchers:

Identify the glycans binding partners in biological samples
Identify whether target proteins are carbohydrate binding proteins
Probe binding of viruses and whole cells to glycans^1-3
Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.)^4-6
Profile the inflammatory immune response

The 100 synthetic glycans featured in the Glycan Array 100 are the most frequently identified structures showing important binding function in the literature. For example, influenza virus binds to a variety of sialosides with a serotype-specific pattern.^7-9 Galectins, which are involved in apoptosis, cell adhesion and T-cell activation suppression, function by binding beta-galactosides.^10-12

Glycan Names (100): Click Here for the Glycan Chart

β-Glc-	β-Gal-	α-Man-	α-Fuc-
α-Rha-	β-GlcNAc-	β-GalNAc-	Tobramycin
Gal-β-1,3-GlcNAc-β-	Gal-α-1,3-Gal-β-1,3-GlcNAc-β-	Neu5Ac-α-2,3-Gal-β-1,3-GlcNAc-β-	Neu5Ac-α-2,6-Gal-β-1,3-GlcNAc-β-
Neu5Gc-α-2,3-Gal-β-1,3-GlcNAc-β-	Neu5Gc-α-2,6-Gal-β-1,3-GlcNAc-β-	Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Lewis A]	Gal-β-1,4-Glc-β-
Gal-α-1,3-Gal-β-1,4-Glc-β-	Gal-α-1,4-Gal-β-1,4-Glc-β-	GlcNAc-β-1,3-Gal-β-1,4-Glc-β-	GalNAc-β-1,3-Gal-β-1,4-Glc-β-
Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-	Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-	Neu5Gc-α-2,3-Gal-β-1,4-Glc-β-	Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-
Gal-β-1,4-(Fuc-α-1,3)-Glc-β-	GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-β-	GlcNAc-β-1,6-GlcNAc-β-	4-P-GlcNAc-β-1,4-Man-β-.
Glc-α-1,2-Gal-α-1,3-Glc-α-	Gal-β-1,3-GalNAc-α-	Gal-β-1,4-GlcNAc-β-	Gal-β-1,4 -(Fuc-α-1,3)-GlcNAc-β- [Lewis X]
Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [Sialyl Lewis X]	Neu5Ac-α-2,3-Gal-β-1,3 -(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A]	Neu5Gc-α-2,3-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A]	Gal-α-1,4-Gal-β-1,3-GlcNAc-β-
Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNnT]	GlcA-β-1,4-GlcNAc-α-1,4-GlcA-β-	GlcNAc-β-1,6-(Gal-β-1,3)-GalNAc-α-O-Ser	Neu5Ac-α-2,3Gal-β-1,4-(6S)GlcNAc-β-
GalNAc-β-1,4-GlcNAc-β-	Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal β-1,4-Glc-β-	Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-	GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood A antigen tetrose]
GlcNAc-β-1,2-Man-α-	Neu5Ac-α-2,3-Gal-β-	Gal-β-1,3 -GalNAc-β-1,3-Gal-β-	Glc-α-1,2-Gal-α-
Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β-1,3-Gal-β-	Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-Glc-β- [3-Sialyl-3-fucosyllactose/ F-SL]	GlcNAc-β-1,4-GlcNAc-β-	β-D-GlcA-
Gal-β-1,4-(6S)GlNAc-β-	GlcNAc-α-1,3-(Glc-α-1,2-Glc-α-1,2)-Gal-α-1,3-Glc-α-	Gal-β-1,3-GalNAc-β-1,4-(Neu5Gc-α-2,3)-Gal-β-1,4-Glc-β-	Sisomicin Sulfate
GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood A antigen trisaccharide]	Fuc-α-1,2-Gal-β-1,4-GlcNAc-β- [Blood H antigen trisaccharide]	Gal-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood B antigen trisaccharide]	Fuc-α-1,2-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNFP I]
Fuc-α-1,2-Gal-β-1,4-Glc-β- [Blood H antigen trisaccharide]	Gal-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood B antigen tetrasaccharide]	(Fuc-α-1,2)-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [ Lewis Y]	(Fuc-α-1,2)-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [ Lewis B]
Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,4)-Glc-β- [Lewis A]	Gal-β-1,3-GalNAc-β-	Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-	Neu5Ac-α-2,6-Gal-β-1,3-GalNAc-β-
Neu5Ac-α-2,6-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-	Neu5Ac-α-2,3-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-	Neu5Ac-α-2,6-(Neu5Ac-α-2,3)-Gal-β-1,3-GalNAc-β-	GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GM2]
GalNAc-β-1,4-(Neu5Ac-α-2,8-Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GD2]	Gal-α-1,4-Gal-β-1,4-GlcNAc-β-	β-D-Rha-	Glc-α-1,4-Glc-β-
Glc-α-1,6-Glc-α-1,4-Glc-β-	Maltotriose-β-	Glc-α-1,6-Glc-α-1,6-Glc-β-	Maltotetraose-β-
GlcNAc-α-1,4-GlcA-β-1,4-GlcNAc-α1,4-GlcA-β-	Maltohexaose-β-	Maltoheptaose-β-	Acarbose-β-
D-pentamannuronic acid-β-	L-pentaguluronic acid-β-	D-cellose-β-	Gal-α-1,3-Gal-β-
β-1,4-Xylotetrose	Chitin-trisaccharide	KDN-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-	Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-Glc-β-
Neu5Ac-α-2,8-Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-	Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-Glc-	Gal-β-1,3-GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β-	Gentamicin Sulfate
Kanamycin sulfate	Geneticin Disulfate Salt (G418)	Neomycin trisulfate	SGP

Application Data

Kit Components

Dialysis Vials
Labeling Reagent
Stop Solution
Glycan Array Glass Slide Assembly
Sample Diluent
20X Wash Buffer I
20X Wash Buffer II
Cy3 equivalent dye-conjugated Streptavidin
Slide Washer/Dryer
Adhesive device sealer
Labeling Buffer
Floating Dialysis Rack

Other Materials Required

Detection antibodies of interest (For sandwich-based method only)
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Distilled or de-ionized water
1.5ml Polypropylene microcentrifuge tubes
Gene microarray scanner or similar laser fluorescence scanner
Small plastic or glass containers
KCl, NaCl, KH2PO4 and Na2HPO4 (For label-based method only)
Beaker, stir plate and stir bar

Protocol Outline

Dry the glass slide
Block array surfaces
Incubate samples (samples need to be biotinylated for the label-based approach)
For the the sandwich-based principle, incubate with a detection antibody cocktail
For the label-based principle, incubate the labeled-streptavidin.
Incubate with Cy3 Equivalent Dye-Streptavidin
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the RayBiotech Glycan Array 100 kit should be stored at -20°C. After initial use, remaining reagents should be stored at 4°C to avoid repeated freeze-thaw cycles and may be stored for up to 3 months (Labeling Reagent, Item B, should be prepared fresh each time before use). Unused glass slides should be kept at -20°C and repeated freeze-thaw cycles should be avoided (slides may be stored for up to 6 months). The entire kit should be used within 6 months of purchase.

References

Amonsen, M.; Smith, D. F.; Cummings, R. D.; Air, G. M. J Virol 2007, 81, 8341-8345.
Paulson, J. C.; de Vries, R. P. Virus Res 2013, 178, 99-113.
Stencel-Baerenwald, J. E.; Reiss, K.; Reiter, D. M.; Stehle, T.; Dermody, T. S. Nat Rev Microbiol 2014, 12, 739-749.
Park, S.; Shin, I. Org Lett 2007, 9, 1675-1678.
Pourceau, G.; Chevolot, Y.; Goudot, A.; Giroux, F.; Meyer, A.; Moules, V.; Lina, B.; Cecioni, S.; Vidal, S.; Yu, H.; Chen, X.; Ferraris, O.; Praly, J. P.; Souteyrand, E.; Vasseur, J. J.; Morvan, F. Chembiochem 2011, 12, 2071-2080.
Laurent, N.; Voglmeir, J.; Flitsch, S. L. Chem Commun 2008, 4400-4412.
Xu, R.; de Vries, R. P.; Zhu, X.; Nycholat, C. M.; McBride, R.; Yu, W.; Paulson, J. C.; Wilson, I. A. Science 2013, 342, 1230-1235.
Xu, R.; McBride, R.; Nycholat, C. M.; Paulson, J. C.; Wilson, I. A. J Virol 2012, 86, 982-990.
Stevens, J.; Chen, L. M.; Carney, P. J.; Garten, R.; Foust, A.; Le, J.; Pokorny, B. A.; Manojkumar, R.; Silverman, J.; Devis, R.; Rhea, K.; Xu, X.; Bucher, D. J.; Paulson, J. C.; Cox, N. J.; Klimov, A.; Donis, R. O. J Virol 2010, 84, 8287-8299.
Stowell, S. R.; Arthur, C. M.; Mehta, P.; Slanina, K. A.; Blixt, O.; Leffler, H.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 10109-10123.
Stowell, S. R.; Arthur, C. M.; Slanina, K. A.; Horton, J. R.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 20547-20559.
Carlsson, S.; Oberg, C. T.; Carlsson, M. C.; Sundin, A.; Nilsson, U. J.; Smith, D.; Cummings, R. D.; Almkvist, J.; Karlsson, A.; Leffler, H. Glycobiology 2007, 17, 663-676.