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Glycan Array 100

Detection of binding partners for 100 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.

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GA-Glycan-100-1
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Assay Format

Number of Targets Detected:
100
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Purified Proteins
  • Serum
  • Tissue Lysates
Design Principle:
  • Label-based
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4, 8, or 16 Sample Kit

Introduction

Glycocalyx, literally meaning ‘sugar coat’, is an extracellular polymeric coating surrounding many prokaryotic and eukaryotic cells consisting of glycoproteins, glycolipids, proteoglycans and glycosaminoglycans. The constituents of the glycocalyx play an important role in the process of cell signaling, virus infections, and immunity. However, detection tools for the research of glycobiology are currently in very limited supply.

Raybiotech has pioneered the development of antibody arrays which are now widely applied in the research community with thousands of peer reviewed publications, including Cell and Nature. Taking advantage of advancements in microarray technology developed for antibody arrays, we have developed the first commercially available glycan array for screening protein-carbohydrate interactions. This array will help researchers:

  • Identify the glycans binding partners in biological samples
  • Identify whether target proteins are carbohydrate binding proteins
  • Probe binding of viruses and whole cells to glycans1-3
  • Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.)4-6
  • Profile the inflammatory immune response


The 100 synthetic glycans featured in the Glycan Array 100 are the most frequently identified structures showing important binding function in the literature. For example, influenza virus binds to a variety of sialosides with a serotype-specific pattern.7-9 Galectins, which are involved in apoptosis, cell adhesion and T-cell activation suppression, function by binding beta-galactosides.10-12

Glycan Names (100): Click Here for the Glycan Chart

β-Glc-β-Gal-α-Man-α-Fuc-
α-Rha-β-GlcNAc-β-GalNAc-Tobramycin
Gal-β-1,3-GlcNAc-β-Gal-α-1,3-Gal-β-1,3-GlcNAc-β-Neu5Ac-α-2,3-Gal-β-1,3-GlcNAc-β-Neu5Ac-α-2,6-Gal-β-1,3-GlcNAc-β-
Neu5Gc-α-2,3-Gal-β-1,3-GlcNAc-β-Neu5Gc-α-2,6-Gal-β-1,3-GlcNAc-β-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Lewis A]Gal-β-1,4-Glc-β-
Gal-α-1,3-Gal-β-1,4-Glc-β-Gal-α-1,4-Gal-β-1,4-Glc-β-GlcNAc-β-1,3-Gal-β-1,4-Glc-β-GalNAc-β-1,3-Gal-β-1,4-Glc-β-
Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-Neu5Gc-α-2,3-Gal-β-1,4-Glc-β-Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-
Gal-β-1,4-(Fuc-α-1,3)-Glc-β-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-β-GlcNAc-β-1,6-GlcNAc-β-4-P-GlcNAc-β-1,4-Man-β-.
Glc-α-1,2-Gal-α-1,3-Glc-α-Gal-β-1,3-GalNAc-α-Gal-β-1,4-GlcNAc-β-Gal-β-1,4 -(Fuc-α-1,3)-GlcNAc-β- [Lewis X]
Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [Sialyl Lewis X]Neu5Ac-α-2,3-Gal-β-1,3 -(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A]Neu5Gc-α-2,3-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A]Gal-α-1,4-Gal-β-1,3-GlcNAc-β-
Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNnT]GlcA-β-1,4-GlcNAc-α-1,4-GlcA-β-GlcNAc-β-1,6-(Gal-β-1,3)-GalNAc-α-O-SerNeu5Ac-α-2,3Gal-β-1,4-(6S)GlcNAc-β-
GalNAc-β-1,4-GlcNAc-β-Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal β-1,4-Glc-β-Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood A antigen tetrose]
GlcNAc-β-1,2-Man-α-Neu5Ac-α-2,3-Gal-β-Gal-β-1,3 -GalNAc-β-1,3-Gal-β-Glc-α-1,2-Gal-α-
Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β-1,3-Gal-β-Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-Glc-β- [3-Sialyl-3-fucosyllactose/ F-SL]GlcNAc-β-1,4-GlcNAc-β-β-D-GlcA-
Gal-β-1,4-(6S)GlNAc-β-GlcNAc-α-1,3-(Glc-α-1,2-Glc-α-1,2)-Gal-α-1,3-Glc-α-Gal-β-1,3-GalNAc-β-1,4-(Neu5Gc-α-2,3)-Gal-β-1,4-Glc-β-Sisomicin Sulfate
GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood A antigen trisaccharide]Fuc-α-1,2-Gal-β-1,4-GlcNAc-β- [Blood H antigen trisaccharide]Gal-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood B antigen trisaccharide]Fuc-α-1,2-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNFP I]
Fuc-α-1,2-Gal-β-1,4-Glc-β- [Blood H antigen trisaccharide]Gal-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood B antigen tetrasaccharide](Fuc-α-1,2)-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [ Lewis Y](Fuc-α-1,2)-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [ Lewis B]
Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,4)-Glc-β- [Lewis A]Gal-β-1,3-GalNAc-β-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-Neu5Ac-α-2,6-Gal-β-1,3-GalNAc-β-
Neu5Ac-α-2,6-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-Neu5Ac-α-2,3-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-Neu5Ac-α-2,6-(Neu5Ac-α-2,3)-Gal-β-1,3-GalNAc-β-GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GM2]
GalNAc-β-1,4-(Neu5Ac-α-2,8-Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GD2]Gal-α-1,4-Gal-β-1,4-GlcNAc-β-β-D-Rha-Glc-α-1,4-Glc-β-
Glc-α-1,6-Glc-α-1,4-Glc-β-Maltotriose-β-Glc-α-1,6-Glc-α-1,6-Glc-β-Maltotetraose-β-
GlcNAc-α-1,4-GlcA-β-1,4-GlcNAc-α1,4-GlcA-β-Maltohexaose-β-Maltoheptaose-β-Acarbose-β-
D-pentamannuronic acid-β-L-pentaguluronic acid-β-D-cellose-β-Gal-α-1,3-Gal-β-
β-1,4-XylotetroseChitin-trisaccharideKDN-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-Glc-β-
Neu5Ac-α-2,8-Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-Glc-Gal-β-1,3-GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β-Gentamicin Sulfate
Kanamycin sulfateGeneticin Disulfate Salt (G418)Neomycin trisulfateSGP

Application Data

Kit Components
  • Dialysis Vials
  • Labeling Reagent
  • Stop Solution
  • Glycan Array Glass Slide Assembly
  • Sample Diluent
  • 20X Wash Buffer I
  • 20X Wash Buffer II
  • Cy3 equivalent dye-conjugated Streptavidin
  • Slide Washer/Dryer
  • Adhesive device sealer
  • Labeling Buffer
  • Floating Dialysis Rack
Other Materials Required
  • Detection antibodies of interest (For sandwich-based method only)
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Distilled or de-ionized water
  • 1.5ml Polypropylene microcentrifuge tubes
  • Gene microarray scanner or similar laser fluorescence scanner
  • Small plastic or glass containers
  • KCl, NaCl, KH2PO4 and Na2HPO4 (For label-based method only)
  • Beaker, stir plate and stir bar
Protocol Outline
  1. Dry the glass slide
  2. Block array surfaces
  3. Incubate samples (samples need to be biotinylated for the label-based approach)
  4. For the the sandwich-based principle, incubate with a detection antibody cocktail
    For the label-based principle, incubate the labeled-streptavidin.
  5. Incubate with Cy3 Equivalent Dye-Streptavidin
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the RayBiotech Glycan Array 100 kit should be stored at -20°C. After initial use, remaining reagents should be stored at 4°C to avoid repeated freeze-thaw cycles and may be stored for up to 3 months (Labeling Reagent, Item B, should be prepared fresh each time before use). Unused glass slides should be kept at -20°C and repeated freeze-thaw cycles should be avoided (slides may be stored for up to 6 months). The entire kit should be used within 6 months of purchase.

References

  1. Amonsen, M.; Smith, D. F.; Cummings, R. D.; Air, G. M. J Virol 2007, 81, 8341-8345.
  2. Paulson, J. C.; de Vries, R. P. Virus Res 2013, 178, 99-113.
  3. Stencel-Baerenwald, J. E.; Reiss, K.; Reiter, D. M.; Stehle, T.; Dermody, T. S. Nat Rev Microbiol 2014, 12, 739-749.
  4. Park, S.; Shin, I. Org Lett 2007, 9, 1675-1678.
  5. Pourceau, G.; Chevolot, Y.; Goudot, A.; Giroux, F.; Meyer, A.; Moules, V.; Lina, B.; Cecioni, S.; Vidal, S.; Yu, H.; Chen, X.; Ferraris, O.; Praly, J. P.; Souteyrand, E.; Vasseur, J. J.; Morvan, F. Chembiochem 2011, 12, 2071-2080.
  6. Laurent, N.; Voglmeir, J.; Flitsch, S. L. Chem Commun 2008, 4400-4412.
  7. Xu, R.; de Vries, R. P.; Zhu, X.; Nycholat, C. M.; McBride, R.; Yu, W.; Paulson, J. C.; Wilson, I. A. Science 2013, 342, 1230-1235.
  8. Xu, R.; McBride, R.; Nycholat, C. M.; Paulson, J. C.; Wilson, I. A. J Virol 2012, 86, 982-990.
  9. Stevens, J.; Chen, L. M.; Carney, P. J.; Garten, R.; Foust, A.; Le, J.; Pokorny, B. A.; Manojkumar, R.; Silverman, J.; Devis, R.; Rhea, K.; Xu, X.; Bucher, D. J.; Paulson, J. C.; Cox, N. J.; Klimov, A.; Donis, R. O. J Virol 2010, 84, 8287-8299.
  10. Stowell, S. R.; Arthur, C. M.; Mehta, P.; Slanina, K. A.; Blixt, O.; Leffler, H.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 10109-10123.
  11. Stowell, S. R.; Arthur, C. M.; Slanina, K. A.; Horton, J. R.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 20547-20559.
  12. Carlsson, S.; Oberg, C. T.; Carlsson, M. C.; Sundin, A.; Nilsson, U. J.; Smith, D.; Cummings, R. D.; Almkvist, J.; Karlsson, A.; Leffler, H. Glycobiology 2007, 17, 663-676.
Manual SDS
  1. Hwang H., Han J., Kim G., Han J. Functional Expression and Characterization of the Recombinant N-Acetyl-Glucosamine/N-Acetyl-Galactosamine-Specific Marine Algal Lectin BPL3. Mar Drugs. 2018 Jan 5;16(1). pii: E13. doi: 10.3390/md16010013.
    Species: N/A
    Sample type: Purified Proteins
  2. Dan X., Liu W., Ng T. Development and Applications of Lectins as Biological Tools in Biomedical Research. Med Res Rev. 2016 Mar;36(2):221-47. doi: 10.1002/med.21363.
    Species: N/A
    Sample type: N/A
  3. McCombs J., Diaz J., Luebke K., Kohler J. Glycan specificity of neuraminidases determined in microarray format. Carbohydr Res. 2016 Jun 16;428:31-40. doi: 10.1016/j.carres.2016.04.003.
    Species: N/A
    Sample type: Neuraminidase Proteins

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