Glycan Array 300

$1,149.53
GA-Glycan-300-1

Detection of binding partners for 300 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.

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Assay Format

Number of Targets Detected:
300
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Purified Proteins
  • Serum
  • Tissue Lysates
Design Principle:
  • Label-based
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4, 8, or 16 Sample Kit

Introduction

Glycocalyx, literally meaning ‘sugar coat’, is an extracellular polymeric coating surrounding many prokaryotic and eukaryotic cells consisting of glycoproteins, glycolipids, proteoglycans and glycosaminoglycans. The constituents of the glycocalyx play an important role in the process of protein folding and trafficking, cell signaling and intercellular interactions, cell pathogen interactions, and immunity. However, detection tools for the research of glycobiology are currently in very limited supply.

 

Raybiotech has pioneered the development of antibody arrays which are now widely applied in the research community with thousands of peer reviewed publications, including in Cell and Nature. Taking advantage of advancements in microarray technology developed for antibody arrays, we have developed a new generation glycan array, the Glycan-300, soon after previously launching the Glycan-100. The Glycan-300 is currently the largest commercially available glycan array for screening protein-carbohydrate interactions. This array will help researchers:

  1. Identify glycan binding partners in biological samples
  2. Identify whether target proteins are carbohydrate binding proteins
  3. Probe binding of pathogens including bacteria, viruses and whole cells to glycans1-3
  4. Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.)4-6
  5. Profile the inflammatory and/or immune response.

 

The 300 synthetic glycans featured in the new generation Glycan Glycan-300 array consists of four groups of targets including the 100 glycans from the Raybio Glycan-100 array and an additional 80 N-glycans, 50 glycolipid glycans, and 50 human milk oligosaccharide glycans. The included glycans have been frequently identified as structures showing important binding functions in the literature. For example, a variety of sialosides have been shown to bind to the influenza virus with a serotype-specific pattern.7-9 Galectins, which are involved in apoptosis, cell adhesion and T-cell activation suppression, function by binding beta-galactosides.10-12 Glycosphingolipids interact with growth factor receptors to modify signal transduction or with bacterial toxin subunits, such as Vero(Shiga) toxin, cholera toxin, and the heat labile E.coli toxins, to induce cell apoptosis13. Additionally, oligosaccharides from human milk bind to microbes to promote growth of normal flora and inhibit pathogens in the gastrointestinal tract14. This array provides a powerful tool for researchers in glycobiology and other fields.

Glycan Names: Download Full Glycan List | Download Glycan Structures

 

N-Glycans

Man-α-1,6-(Man-α-1,3-)Man-α-1,6-(GlcNAc-β-1,2-Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(Man-α-1,3-)Man-α-1,6-(Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(Man-α-1,3-)Man-α-1,6-(Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-) Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(Man-α-1,3-)Man-α-1,6-(Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-) Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5
Man-α-1,6-(Man-α-1,3-)Man-α-1,6-[Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(Man-α-1,3-)Man-α-1,6-[Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2- Man-α-1,3-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5
Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5
Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Gc-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5
Man-α-1,6-(GlcNAc-β-1,2-Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 GlcNAc-β-1,2-Man-α-1,6-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5
Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5
Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5
Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 GlcNAc-β-1,2-Man-α-1,6-[GlcNAc(3Ac)-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5
 

Glycolipid Glycans

Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,3-Gal-β-1,4-Glc-Sp5 Kdn-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-(GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5
Neu5Gc-a-2,3-(GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Kdn-a-2,3-(GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Neu5Ac-a-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Neu5Gc-a-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5
Kdn-a-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp5
Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Gc-α-2-,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,8-Kdn-α-2,3-Gal-β-1,4-Glc-Sp5
Kdn-α-2,8-Kdn-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,8-Neu5Ac-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Ac-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Ac-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5
Neu5Ac-α-2,8-Neu5Gc-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Gc-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Gc-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Kdn-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5
Neu5Ac-α-2,8-Neu5Ac-α-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Ac-α-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Gc-α-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Gc-α-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5
GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-a-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-a-1,4-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-a-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
Kdn-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,8-Neu5Ac-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,8-Neu5Ac-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,8-Neu5Gc-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
Neu5Gc-a-2,8-Neu5Gc-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-a-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
Gal-a-1,4-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
Neu5Gc-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
Neu5Gc-a-2,3-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-α-1,4-Gal-β-1,4-Glc-Sp5 GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5
Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5
Gal-α-1,3-Gal-β-1,4-Glc-Sp5 GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5
Neu5Gc-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5
 

Human Milk Oligosaccharides

GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,3-(Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5
Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-[Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,6-]Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5
Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5
Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-[Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,6-]Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5
GlcNAc-β-1,3-(Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-[Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,6-]Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-(Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5
Gal-β-1,4-GlcNAc-β-1,3-(Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-[Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,6-]Gal-β-1,4-Glc-Sp5 Gal-β-1,4-Glc-Sp GalNAc-β-1,3-Gal-β-1,4-Glc-Sp
Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp Neu5Gc-α-2,3-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,6-Gal-β-1,4-Glc-Sp Neu5Gc-α-2,6-Gal-β-1,4-Glc-Sp
Gal-α-1,3-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp
Neu5Ac-α-2,6-Gal-α-1,3-Gal-β-1,4-Glc-Sp Gal-α-1,4-Gal-β-1,4-Glc-Sp5 GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5
Fuc-α-1,2-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-(Neu5Ac-α-2,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5
Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,3-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5
 

Tandem Epitopes

Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-Man-α-1,3-( Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,8-Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,8-Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,8-Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,8-Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Neu5Ac-α-2,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Neu5Ac-α-2,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Neu5Gc-α-2,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5AC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-[Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5AC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5GC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-[Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5GC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-(Neu5AC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-[Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-(Neu5AC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Gal-α-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
Gal-α-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn
 

Glycan 100 Glycans

β-Glc-Sp β-Gal-Sp α-Man-Sp α-Fuc-Sp
α-Rha-Sp β-GlcNAc-Sp β-GalNAc-Sp Tobramycin
Gal-β-1,3-GlcNAc-β-Sp Gal-α-1,3-Gal-β-1,3-GlcNAc-β-Sp Neu5Ac-α-2,3-Gal-β-1,3-GlcNAc-β-Sp Neu5Ac-α-2,6-Gal-β-1,3-GlcNAc-β-Sp
Neu5Gc-α-2,3-Gal-β-1,3-GlcNAc-β-Sp Neu5Gc-α-2,6-Gal-β-1,3-GlcNAc-β-Sp Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Lewis A] –Sp Gal-β-1,4-Glc-β-Sp
Gal-α-1,3-Gal-β-1,4-Glc-β-Sp Gal-α-1,4-Gal-β-1,4-Glc-β-Sp GlcNAc-β-1,3-Gal-β-1,4-Glc-β-Sp GalNAc-β-1,3-Gal-β-1,4-Glc-β-Sp
Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Sp Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-Sp Neu5Gc-α-2,3-Gal-β-1,4-Glc-β-Sp Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-Sp
Gal-β-1,4-(Fuc-α-1,3)-Glc-β-Sp GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-β-Sp GlcNAc-β-1,6-GlcNAc-β-Sp 4-P-GlcNAc-b-1,4-Man-b-Sp
Glc-α-1,2-Gal-α-1,3-Glc-α-Sp Gal-β-1,3-GalNAc-α-Sp Gal-β-1,4-GlcNAc-β-Sp Gal-β-1,4 -(Fuc-α-1,3)-GlcNAc-β- [Lewis X] –Sp
Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [Sialyl Lewis X]-Sp Neu5Ac-α-2,3-Gal-β-1,3 -(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A]-Sp Neu5Gc-α-2,3-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A]-Sp Gal-α-1,4-Gal-β-1,3-GlcNAc-β-Sp
Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNnT]-Sp GlcA-β-1,4-GlcNAc-α-1,4-GlcA-β-Sp GlcNAc-β-1,6-(Gal-β-1,3)-GalNAc-α-O-Ser- Sp4 Neu5Ac-α-2,3Gal-β-1,4-(6S)GlcNAc-β-Sp
GalNAc-β-1,4-GlcNAc-β-Sp2 Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal β-1,4-Glc-β-Sp Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Sp GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood A antigen tetrose]-Sp1
GlcNAc-β-1,2-Man-α-Sp Neu5Ac-α-2,3-Gal-β-Sp1 Gal-β-1,3 -GalNAc-β-1,3-Gal-β-Sp1 Glc-α-1,2-Gal-α-Sp
Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β-1,3-Gal-β-Sp1 Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-Glc-β- [3-Sialyl-3-fucosyllactose/ F-SL]-Sp1 GlcNAc-β-1,4-GlcNAc-β-Sp1 β-D-GlcA-Sp
Gal-β-1,4-(6S)GlNAc-β-Sp GlcNAc-α-1,3-(Glc-α-1,2-Glc-α-1,2)-Gal-α-1,3-Glc-α-Sp Gal-β-1,3-GalNAc-β-1,4-(Neu5Gc-α-2,3)-Gal-β-1,4-Glc-β-Sp1 Sisomicin Sulfate
GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood A antigen trisaccharide]-Sp1 Fuc-α-1,2-Gal-β-1,4-GlcNAc-β- [Blood H antigen trisaccharide]-Sp1 Gal-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood B antigen trisaccharide]-Sp1 Fuc-α-1,2-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNFP I]-Sp1
Fuc-α-1,2-Gal-β-1,4-Glc-β- [Blood H antigen trisaccharide]-Sp1 Gal-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood B antigen tetrasaccharide]-Sp1 (Fuc-α-1,2)-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [ Lewis Y]-Sp1 (Fuc-α-1,2)-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [ Lewis B]-Sp1
Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,4)-Glc-β- [Lewis A]-Sp1 Gal-β-1,3-GalNAc-β-Sp1 Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-Sp Neu5Ac-α-2,6-Gal-β-1,3-GalNAc-β-Sp
Neu5Ac-α-2,6-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-Sp Neu5Ac-α-2,3-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-Sp Neu5Ac-α-2,6-(Neu5Ac-α-2,3)-Gal-β-1,3-GalNAc-β-Sp GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GM2]-Sp
GalNAc-β-1,4-(Neu5Ac-α-2,8-Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GD2]-Sp Gal-α-1,4-Gal-β-1,4-GlcNAc-β-Sp1 β-D-Rha-Sp Glc-α-1,4-Glc-β-Sp1
Glc-α-1,6-Glc-α-1,4-Glc-β-Sp1 Maltotriose-β-Sp1 Glc-α-1,6-Glc-α-1,6-Glc-β-Sp1 Maltotetraose-β-Sp1
GlcNAc-α-1,4-GlcA-β-1,4-GlcNAc-α1,4-GlcA-β-Sp Maltohexaose-β-Sp1 Maltoheptaose-β-Sp1 Acarbose-β-Sp1
D-pentamannuronic acid-β-Sp1 L-pentaguluronic acid-β-Sp1 D-cellose-β-Sp1 Gal-α-1,3-Gal-β-Sp1
β-1,4-Xylotetrose-Sp1 Chitin-trisaccharide-Sp1 KDN-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Sp Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-Glc-β-Sp
Neu5Ac-α-2,8-Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Sp3 Neu5Ac-a-2,8-Neu5Ac-a-2,6-Gal-b-1,4-Glc-Sp5 Gal-β-1,3-GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β-Sp1 Gentamicin Sulfate
Kanamycin sulfate Geneticin Disulfate Salt (G418) Neomycin trisulfate SGP

Application Data

Kit Components
  • Dialysis Vials
  • Labeling Reagent
  • Stop Solution
  • Glycan Array Glass Slide Assembly
  • Sample Diluent
  • 20X Wash Buffer I
  • 20X Wash Buffer II
  • Cy3 equivalent dye-conjugated Streptavidin
  • Slide Washer/Dryer
  • Adhesive device sealer
  • Labeling Buffer
  • Floating Dialysis Rack
Other Materials Required
  • Detection antibodies of interest (For sandwich-based method only)
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Distilled or de-ionized water
  • 1.5ml Polypropylene microcentrifuge tubes
  • Gene microarray scanner or similar laser fluorescence scanner
  • Small plastic or glass containers
  • KCl, NaCl, KH2PO4 and Na2HPO4 (For label-based method only)
  • Beaker, stir plate and stir bar
Protocol Outline
  1. Dry the glass slide
  2. Block array surfaces
  3. Incubate samples (samples need to be biotinylated for the label-based approach)
  4. For the the sandwich-based principle, incubate with a detection antibody cocktail
    For the label-based principle, incubate the labeled-streptavidin.
  5. Incubate with Cy3 Equivalent Dye-Streptavidin
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the RayBiotech Glycan Array 300 kit should be stored at -20°C. After initial use, remaining reagents should be stored at 4°C to avoid repeated freeze-thaw cycles and may be stored for up to 3 months (Labeling Reagent, Item B, should be prepared fresh each time before use). Unused glass slides should be kept at -20°C and repeated freeze-thaw cycles should be avoided (slides may be stored for up to 6 months). The entire kit should be used within 6 months of purchase.

References

  1. Amonsen, M.; Smith, D. F.; Cummings, R. D.; Air, G. M. J Virol 2007, 81, 8341-8345.
  2. Paulson, J. C.; de Vries, R. P. Virus Res 2013, 178, 99-113.
  3. Stencel-Baerenwald, J. E.; Reiss, K.; Reiter, D. M.; Stehle, T.; Dermody, T. S. Nat Rev Microbiol 2014, 12, 739-749.
  4. Park, S.; Shin, I. Org Lett 2007, 9, 1675-1678.
  5. Pourceau, G.; Chevolot, Y.; Goudot, A.; Giroux, F.; Meyer, A.; Moules, V.; Lina, B.; Cecioni, S.; Vidal, S.; Yu, H.; Chen, X.; Ferraris, O.; Praly, J. P.; Souteyrand, E.; Vasseur, J. J.; Morvan, F. Chembiochem 2011, 12, 2071-2080.
  6. Laurent, N.; Voglmeir, J.; Flitsch, S. L. Chem Commun 2008, 4400-4412.
  7. Xu, R.; de Vries, R. P.; Zhu, X.; Nycholat, C. M.; McBride, R.; Yu, W.; Paulson, J. C.; Wilson, I. A. Science 2013, 342, 1230-1235.
  8. Xu, R.; McBride, R.; Nycholat, C. M.; Paulson, J. C.; Wilson, I. A. J Virol 2012, 86, 982-990.
  9. Stevens, J.; Chen, L. M.; Carney, P. J.; Garten, R.; Foust, A.; Le, J.; Pokorny, B. A.; Manojkumar, R.; Silverman, J.; Devis, R.; Rhea, K.; Xu, X.; Bucher, D. J.; Paulson, J. C.; Cox, N. J.; Klimov, A.; Donis, R. O. J Virol 2010, 84, 8287-8299.
  10. Stowell, S. R.; Arthur, C. M.; Mehta, P.; Slanina, K. A.; Blixt, O.; Leffler, H.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 10109-10123.
  11. Stowell, S. R.; Arthur, C. M.; Slanina, K. A.; Horton, J. R.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 20547-20559.
  12. Carlsson, S.; Oberg, C. T.; Carlsson, M. C.; Sundin, A.; Nilsson, U. J.; Smith, D.; Cummings, R. D.; Almkvist, J.; Karlsson, A.; Leffler, H. Glycobiology 2007, 17, 663-676.
  13. Lingwood, C. A. Cold Spring Harb Perspect Biol 2011; 3: a004788
  14. Bode, L. Glycobiology 2012, 22, 1147–1162

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