Protein Name & Synonyms:
Diphtheria Toxin
Applications:
Immunohistology - Frozen, ELISA (dilution: 1/20, 1/200), Western Blotting
Concentration (lot specific):
4 mg/ml
Species Reactivity:
Bacterial
This product recognizes diphtheria toxin (DT), secreted by certain strains of Corynebacterium diphtheriae. DT catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kDa, it is then processed to form A and B fragments both of which appear to be required for toxicity.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Supplied as:
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Formulation:
Phosphate buffered saline
Immunogen:
Purified diptheria toxoid
Preservative:
0.002% | Thiomersal |
1% | Bovine Serum Albumin |
Expiration:
12 months from the date of shipment when stored properly.