Goat Anti-Human BID (C-term)

Goat Anti-Human BID (C-term)
CODE:  DS-PB-02244

Antigen Information

Protein Name & Synonyms:
BID
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.3ug/ml), Western Blotting
Concentration:
IgG concentration 0.5mg/ml
Preparation:
Antisera to BID (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared affinity chromatography.
Species Reactivity:
Human

This product detects bands of approximately 26kDa and 24kDa in A431 cell lysates.

Specificity

This product detects an epitope within the C-terminus of BID, a death agonist that heterodimerizes with other members of the Bcl-2 family of cell death regulators; including the agonist BAX or antagonist BCL2.

BID contains a BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Protease-cleaved BID translocates to mitochondria where it results in cytochrome c release, leading to caspase activation and cell death. This constitutes the activation of the mitochondria death pathway (the intrinsic pathway) by the death stimuli.

BID has been shown to promote cell cycle progression into S phase, whilst BID could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. Deletion of BID inhibits carcinogenesis in the liver, and promotes tumorigenesis in the myeloid cells.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
TRIS buffered saline
Format Type:
Purified

Preparation

Antisera to BID (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared affinity chromatography.

Immunogen:
Peptide with sequence C-TYVRSLARNGMD from the C-terminus of BID
Preservative:
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin