Goat Anti-Human CD195 (N-term.)

Goat Anti-Human CD195 (N-term.) (0.1 mg)
CODE:  129-10113

Antigen Information

Protein Name & Synonyms:
CD195
Target Species:
  • Human

Antibody Specifications

Applications:
Immunohistology - Frozen, ELISA (dilution: , 1/1000), Immunohistology - Paraffin, Western Blotting
Concentration:
1.0mg/ml
Host Species:
Goat
Immunogen Sequence:
YQVSSPIYDINYYTSEPCQKINVKQIAA
Species Reactivity:
Human, Macaque

Specificity

This product specifically recognizes an epitope within the N-terminal (NT) region of human CD195, otherwise known as CCR5 (C-C chemokine receptor type 5), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by a subset of memory Th1 (type 1 helper T) cells.

CD195 acts as a high-affinity receptor for several chemotactic chemokines, including MIP-1 alpha (macrophage inflammatory protein 1 alpha), MIP-1 beta, and RANTES (regulated on activation normal T expressed and secreted protein). CD195 is also a major co-receptor for HIV-1 infection, with variations in the CD195 gene, influencing the rate of progression into AIDS.

This product is reported as suitable for use in immunocytochemistry on human peripheral blood leucocytes.

Storage/Stability

Store at -20 °C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antiserum to human CD195 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Immunogen:
Synthetic peptide YQVSSPIYDINYYTSEPCQKINVKQIAA corresponding to amino acids 3-30 within the N-terminal region of human CD195.
Preservative:
0.09% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin