Protein Name & Synonyms:
Immunohistology - Paraffin, Western Blotting
Human, Mouse, Rat
This product specifically recognizes an epitope within the N-terminal (NT) region of CD284, also known as Toll-like receptor 4 (TLR4), a highly conserved member of the toll-like receptor family, expressed by peripheral blood leucocytes, activated T cells, monocytes, macrophages, dendritic cells, granulocytes and endothelial cells, which plays a primary role in the activation of innate immunity.
CD284 acts as a receptor for bacterial lipopolysaccharides (LPS) in co-operation with CD14 and MD2 (LY96), resulting in the activation of the MyD88-dependent and MyD88-independent (TRIF-dependent) signalling pathways and the production of inflammatory cytokines. LPS-activated CD284 can also induce dendritic cell maturation via TRIF-dependent signaling.
This product is reported as suitable for use in immunocytochemistry on acetone fixed cells.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Antiserum to human CD284 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Synthetic peptide LIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYC corresponding to amino acids 161-192 within the N-terminal region of Human CD284.
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin