Goat Anti-Human Creatine Phosphokinase

Goat Anti-Human Creatine Phosphokinase

$347.00
129-10218
+

Antigen Information

Protein Name & Synonyms:
Creatine Phosphokinase
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 1/500, 1/1000), Western Blotting
Concentration:
5.0 mg/ml
Host Species:
Goat
Species Reactivity:
Human

Specificity

Creatine phosphokinase, also known as creatine kinase (CK), is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP).

In cells, the "cytosolic" CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB.

This product recognizes the CKMM isoenzyme and was found to react ~50% with CKMB when tested in ELISA.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to human creatine phosphokinase were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on protein G.

Immunogen:
CKMM from skeletal muscle
Preservative:
0.09%Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.