Goat Anti-Human GAPDH (C-term)

Goat Anti-Human GAPDH (C-term)

DS-PB-00846
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Antigen Information

Protein Name & Synonyms:
GAPDH
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.01, 0.03ug/ml), Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 0.5mg/ml
Preparation:
Antisera to human GAPDH (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, Mouse

This product detects a band of approximately 35kDa in human tonsil, mouse spleen and HeLa cell lysates.

Specificity

This product recognizes an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 36kD multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.

GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
TRIS buffered saline
Format Type:
Purified

Preparation

Antisera to human GAPDH (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Peptide sequence C-HQVVSSDFNSDT corresponding to the C-terminal region of GAPDH (NP_002037.2).
Preservative:
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.