Goat Anti-Human Ku80 / XRCC5

Goat Anti-Human Ku80 / XRCC5

$571.00
DS-PB-02289
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Antigen Information

Protein Name & Synonyms:
Ku80 / XRCC5
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.3ug/ml), Western Blotting
Concentration:
IgG concentration 0.5mg/ml
Preparation:
Antiserum to human Ku80 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Species Reactivity:
Human

This product detects a band of approximately 80-85kDa in HeLa cell lysates. An additional 75kDa band of unknown origin may be observed.

Specificity

This product specifically recognizes the 80kDa subunit of human Ku protein, an evolutionarily conserved nuclear ATP-dependent DNA helicase, involved in a major proportion of DNA repair and in V(D)J recombination.

The Ku protein, originally described as an autoantigen, exists as a tightly associated heterodimer consisting of a 70kDa (Ku70) and 80kDa (Ku80) subunit which binds to DNA double-strand break ends (DSB). DNA bound Ku recruits the large catalytic subunit DNA-PKcs to form the DNA-dependent protein kinase complex DNA-PK, facilitating DNA repair by the non-homologous end-joining (NHEJ) pathway.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
TRIS buffered saline
Format Type:
Purified

Preparation

Antiserum to human Ku80 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Immunogen:
Peptide sequence C-SLAKKDEKTDTLED from the internal region of Ku80 (NP_066964.1).
Preservative:
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.