Goat Anti-Human Osteopontin (C-term)

Goat Anti-Human Osteopontin (C-term)

DS-PB-01526
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Antigen Information

Protein Name & Synonyms:
Osteopontin
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 1.0, 3.0 ug/ml), Western Blotting
Concentration:
IgG concentration 0.5mg/ml
Preparation:
Antisera to osteopontin (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human

This product detects a band of approximately 60kDa in lysates of human thymus, tonsil and spleen. Approximate 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glcoprotein.

Specificity

This product recognizes an epitope within the C-terminal (CT) region of osteopontin, an acid-rich, multidomain, phosphorylated 60kD glycoprotein. Osteopontin is a secreted chemokine-like protein that is a member of the SIBLING family, and is synthesised by many cell types.

Osteopontin is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteoponitn is also thought to be important to cell-matrix interaction, and can bind fibronectin, collagen and calcium.

Osteopontin has been suggested to have a role in atherosclerosis, bone remodelling, angiogenesis, wound healing and tissue injuries.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid, ready to use.
Formulation:
TRIS buffered saline
Format Type:
Purified

Preparation

Antisera to osteopontin (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Peptide with sequence C-DPKSKEEDKHLK, from the C-terminal of osteopontin.
Preservative:
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.