Goat Anti-Human TNF-alpha [+Biotin]

Goat Anti-Human TNF-alpha [+Biotin]

DS-PB-02323
+

Antigen Information

Protein Name & Synonyms:
TNF-alpha / TNF
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting
Concentration:
IgG concentration 0.1mg/ml after reconstitution
Preparation:
Antisera to human TNF-alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Human

Specificity

This product is specific for human Tumour Necrosis Factor alpha (TNF❏❏alpha), a 17.4kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.

TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-КB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended.

Preparation

Antisera to human TNF-alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Recombinant human TNF-alpha
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.