Goat Anti-Human TNF-alpha

Goat Anti-Human TNF-alpha

$571.00
DS-PB-02322
+

Antigen Information

Protein Name & Synonyms:
TNF-alpha / TNF
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0mg/ml after reconstitution
Preparation:
Antisera to human TNF-alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Species Reactivity:
Human
Immunohistology:
*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Sodium citrate buffer pH 6.0 is recommended for this purpose.

Specificity

This product is specific for human Tumour Necrosis Factor alpha (TNF❏❏alpha), a 17.4kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.

TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-КB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Format Type:
Purified

Preparation

Antisera to human TNF-alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Recombinant human TNF-alpha
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.