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Antigen Information

Protein Name & Synonyms:

VDR

Target Species:

Human

Antibody Specifications

Applications:

ELISA (dilution: 0.3, 1.0ug/ml), Western Blotting

Concentration:

IgG concentration 0.5 mg/ml

Preparation:

Antisera to human Vitamin D receptor were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Species Reactivity:

Human

This product detects a band of approximately 40-45kDa in human brain cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Specificity

This product recognizes human vitamin D nuclear hormone receptor (VDR) a 48 kDa member of the trans-acting transcriptional regulatory factors. On binding vitamin D, VDR regulates the expression of hormone sensitive genes. The main downstream target of VDR is involved in bone mineral metabolism although VDR is also involved in immune response and cancer metabolic pathways. VDR also acts as a receptor for secondary bile acid lithocholic acid. Additionally, signalling and oncogenic activity of beta-catenin signalling has been shown to be repressed by VDR.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:

Purified IgG - liquid

Formulation:

TRIS buffered saline

Format Type:

Purified

Preparation

Antisera to human Vitamin D receptor were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Immunogen:

Peptide sequence CGNQDYKYRVSD corresponding to the internal region of Vitamin D receptor (NP_000367.1).

Preservative: