Protein Name & Synonyms:
IL-12
Applications:
ELISA (dilution: 0.10ug/ml, 0.20ug/ml), Western Blotting
Concentration (lot specific):
IgG concentration 50 ug/ml
Preparation:
Antisera to mouse IL-12 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 1.0ml sterile PBS solution containing 0.1% BSA
Species Reactivity:
Mouse
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution.
This product is specific for mouse interleukin-12 (IL-12).
Interleukin-12 is produced by activated lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes and the development of TH1 CD4 +ve cells and CD8 +ve cytotoxic T cells.
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1.0ml sterile PBS solution containing 0.1% BSA
Antisera to mouse IL-12 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Recombinant mouse IL-12
Preservative:
None present
Expiration:
12 months from the date of shipment when stored properly.