Protein Name & Synonyms:
Immunohistology - Frozen, ELISA (dilution: 1/50, 1/1600), Immunohistology - Paraffin
This product detects porcine insulin, which differs from human insulin by one amino acid residue. Insulin is a hormone that functions in carbohydrate homeostasis, and acts to decrease blood glucose concentration by increasing cell permeability to monosaccharides, amino acids and fatty acids, and accelerate glycolysis and glycogen synthesis in the liver.
Insulin is synthesised by the Islets of Langerhans in the pancreas, initially as an inactive precursor called proinsulin. Proinsulin is cleaved by proteases in the cell to form the insulin A chain and B chain, which heterodimerize to form the mature protein.
Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumours of B-cell origin such as insulinoma.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Antisera to pig insulin were raised by repeated immunisations of Guinea Pigs with highly purified antigen. Ig fraction prepared from whole sruem by ammonium sulfate precipitation.