Protein Name & Synonyms:
Immunohistology - Frozen, ELISA (dilution: 1/10 , 1/50), Flow Cytometry, Immunoprecipitation, Western Blotting
IgG concentration 1 mg/ml
This product recognizes mouse and rat CD81, a 26kD cell surface glycoprotein that is also known as TAPA-1. In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation.
Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
38C13, murine B cell line
Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.