Human Adiponectin ELISA

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RayBio® Human Adiponectin (ACRP30) ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-Adiponectin-1
+

Antigen Information

Accession Number:
  • Q15848
Gene ID:
  • 9370
Gene Symbols:
  • ACDC
  • ACRP30
  • ADIPOQ
  • APM1
  • GBP28
Protein Name & Synonyms:
Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipose most abundant gene transcript 1 protein) (apM-1) (Gelatin-binding protein)
Target Species:
  • Human

Assay Format

Detection Range:
25pg/ml - 18,000pg/ml
Recommended Dilution (serum & plasma):
30,000 fold
Sensitivity:
25 pg/ml

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Specificity:
Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
    Species: Human
    Sample type: Serum
  2. Youseef M. The Effect of Dietary Intervention by Low Carbohydrate Diet, and Low Fat Diet, on Weight Loss, Leptin and Adiponectin. Life Science Journal 2015;12(4)
    Species: Human
    Sample type: Serum
  3. Kumar P., Srivastava N., Tiwari S., et al. Correlation of gender and age with serum adipokines level in north indian adults. Asian Journal of Medical Sciences | Sep-Oct 2015 | Vol 6 | Issue 5, 9-11
    Species: Human
    Sample type: Serum
  4. Nabil N., El Sayyad M., et al. moderate exercise training has anorexogenic effect associated with improved oxidative stress in obese women. International Journal of Nutrition and Metabolism. Vol. & (4), pp 52-61, April 2015, DOI: 10.5897/IJNAM2015.0180
    Species: Human
    Sample type: Plasma
  5. Dede N., Ipekci S., Kebapcilar L., et al. Influence of Exercise on Leptin, Adiponectin and Quality of Life in Type 2 Diabetics. Turk J Endocrinol Metab 2015; 19: 7-13
    Species: Human
    Sample type: Serum
  6. Nobili V., Alisi A., Cutrera R., et al. Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver. Thorax. 2015 Aug;70(8):769-81. doi: 10.1136/thoraxjnl-2015-206782
    Species: Human
    Sample type: Serum
  7. Kapil S., Duseja A., Sharma B., et al. Small intestinal bacterial overgrowth and toll-like receptor signaling in patients with non-alcoholic fatty liver disease. J Gastroenterol Hepatol. 2016 Jan;31(1):213-21. doi: 10.1111/jgh.13058.
    Species: Human
    Sample type: Serum
  8. Kalyanasundaram A. Jacob A., Ramachandran H., Sivakumar M. Serum Adiponectin Levels and Their Association with Antiretroviral Therapy and Lipid Profile in HIV-Infected Individuals in South India. Kalyanasundaram et al., J AIDS Clin Res 2015, 6:9
    Species: Human
    Sample type: Serum
  9. Bhaktha G., et al. Adiponectin levels in diabetic subjects without any pre-existing micro and macro vascular complications. Asian J of Biochem and Pharm Res. Issue 2 (Vol. 4) 2014 ISSN: 2231-2560
    Species: Human
    Sample type: Serum
  10. Das P., Bandyopadhyay SK., Biswas S., et al. CORRELATION OF OBESITY AND THE NOVEL ADIPOCYTOKINES, LEPTIN AND ADIPONECTIN WITH INSULIN RESISTANCE IN TYPE 2 DIABETES MELLITUS. Bangladesh J Physiol Pharmacol 2014;30(1):29-32.)
    Species: Human
    Sample type: Serum
Protocall was very user friendly. Good results low background

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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