Human Acrp30 ELISA

$398.00
ELH-Adiponectin-1

RayBio® Human Adiponectin (ACRP30) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
25 pg/ml

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Detection Range
25pg/ml - 18,000pg/ml
Recommended Dilution (serum & plasma)
30,000 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipose most abundant gene transcript 1 protein) (apM-1) (Gelatin-binding protein)
Accession Number
Gene Names
ADIPOQ, ACDC, ACRP30, APM1, GBP28
Gene ID
9370

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • ADIPOQ

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
    Species: Human
    Sample Type: Serum

  2. Youseef M. The Effect of Dietary Intervention by Low Carbohydrate Diet, and Low Fat Diet, on Weight Loss, Leptin and Adiponectin. Life Science Journal 2015;12(4)
    Species: Human
    Sample Type: Serum

  3. Kumar P., Srivastava N., Tiwari S., et al. Correlation of gender and age with serum adipokines level in north indian adults. Asian Journal of Medical Sciences | Sep-Oct 2015 | Vol 6 | Issue 5, 9-11
    Species: Human
    Sample Type: Serum

  4. Nabil N., El Sayyad M., et al. moderate exercise training has anorexogenic effect associated with improved oxidative stress in obese women. International Journal of Nutrition and Metabolism. Vol. & (4), pp 52-61, April 2015, DOI: 10.5897/IJNAM2015.0180
    Species: Human
    Sample Type: Plasma

  5. Dede N., Ipekci S., Kebapcilar L., et al. Influence of Exercise on Leptin, Adiponectin and Quality of Life in Type 2 Diabetics. Turk J Endocrinol Metab 2015; 19: 7-13
    Species: Human
    Sample Type: Serum

  6. Nobili V., Alisi A., Cutrera R., et al. Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver. Thorax. 2015 Aug;70(8):769-81. doi: 10.1136/thoraxjnl-2015-206782
    Species: Human
    Sample Type: Serum

  7. Kapil S., Duseja A., Sharma B., et al. Small intestinal bacterial overgrowth and toll-like receptor signaling in patients with non-alcoholic fatty liver disease. J Gastroenterol Hepatol. 2016 Jan;31(1):213-21. doi: 10.1111/jgh.13058.
    Species: Human
    Sample Type: Serum

  8. Kalyanasundaram A. Jacob A., Ramachandran H., Sivakumar M. Serum Adiponectin Levels and Their Association with Antiretroviral Therapy and Lipid Profile in HIV-Infected Individuals in South India. Kalyanasundaram et al., J AIDS Clin Res 2015, 6:9
    Species: Human
    Sample Type: Serum

  9. Bhaktha G., et al. Adiponectin levels in diabetic subjects without any pre-existing micro and macro vascular complications. Asian J of Biochem and Pharm Res. Issue 2 (Vol. 4) 2014 ISSN: 2231-2560
    Species: Human
    Sample Type: Serum

  10. Das P., Bandyopadhyay SK., Biswas S., et al. CORRELATION OF OBESITY AND THE NOVEL ADIPOCYTOKINES, LEPTIN AND ADIPONECTIN WITH INSULIN RESISTANCE IN TYPE 2 DIABETES MELLITUS. Bangladesh J Physiol Pharmacol 2014;30(1):29-32.)
    Species: Human
    Sample Type: Serum

  11. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print]
    Species: Human
    Sample Type: Plasma

  12. Ha AW., et al. Plasma adipocytokines and antioxidants-status in Korean overweight and obese females with dyslipidemia. Nutr Res Pract. 2014 Aug;8(4):417-424. http://dx.doi.org/10.4162/nrp.2014.8.4.417
    Species: Human
    Sample Type: Plasma

  13. Huang J., et al. Level changes of adiponectin and cd146 in serum in type 2 diabetic nephropathy. Journal of Integrative Nephrology and Andrology, July-September 2014, Vol 1 Issue 1 Page 33 - 37. DOI: 10.4103/2225-1243.137552
    Species: Human
    Sample Type: Serum

  14. Nokhbehsaim M., et al.Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions. Journal of Diabetes Research. Volume 2014, Article ID 796565, 11 pages. http://dx.doi.org/10.1155/2014/796565
    Species: Human
    Sample Type: Conditioned Media

  15. Rathore S., Bidwai A. Association of Insulin Resistance and Selected Adipokines in Cardiovascular Disease with and without Metabolic Syndrome. JMSCR Volume 2, Issue 5, Pages 1176-1186, May 2014
    Species: Human
    Sample Type: Serum

  16. Santovito D., Nardis V., Marcantonio P., et al. Plasma exosome microRNA profiling unravels a new potential modulator of adiponectin pathway in Diabetes: effect of glycemic control. The Journal of Clinical Endocrinology & Metabolism 2014 Epub ahead of print June 17 2014, DOI: http://dx.doi.org/10.1210/jc.2013-3843
    Species: Human
    Sample Type: Plasma

  17. Akuailou EN., et al. Measurement and validation of the nature of salivary adiponectin. Acta Diabetol (2013) 50:727-730, DOI 10.1007/s00592-012-0388-z
    Species: Human
    Sample Type: Saliva

  18. Hoo SP, Loh QL, Yue Z, et al. Preparation of a soft and interconnected macroporous hydroxypropyl cellulose methacrylate scaffold for adipose tissue engineering. J Mater Chem B. 2013 May 20; [Epub ahead of print] doi:10.1039/C3TB00446E
    Species: Human
    Sample Type: Conditioned Media

  19. Hung P., Tsai H., Lin C., Hung K. Abdominal Obesity is Associated with Peripheral Artery Disease in Hemodialysis Patients. PLOS One Published: August 02, 2013 DOI: 10.1371/journal.pone.0069424
    Species: Human
    Sample Type: Serum

  20. Thanakun S., Watanabe H., Thaweboon S., Izumi Y. An effective technique for the processing of saliva for the analysis of leptin and adiponectin. Peptides. 2013 Sep;47:60-5. doi: 10.1016/j.peptides.2013.06.010
    Species: Human
    Sample Type: Saliva

  21. Volberg V., et al. Associations between perinatal factors and adiponectin and leptin in 9-year-old Mexican-American children. Pediatr Obes. 2013 Dec;8(6):454-63. doi: 10.1111/j.2047-6310.2012.00127.x.
    Species: Human
    Sample Type: Plasma

  22. Yanni D, Darendeliler F, Bas F, et al. The role of leptin, soluble leptin receptor, adiponectin and visfatin in insulin sensitivity in preterm born children in prepubertal ages. Cytokine. 2013 May 29; [Epub ahead of print] doi:10.1016/j.cyto.2013.04.034.
    Species: Human
    Sample Type: Serum

  23. Yanni D., et al. The role of leptin, soluble leptin receptor, adiponectin and visfatin in insulin sensitivity in preterm born children in prepubertal ages. Cytokine 64 (2013) 448-453. http://dx.doi.org/10.1016/j.cyto.2013.04.034
    Species: Human
    Sample Type: Serum

  24. Leal V., Lobo J., Stockler-Pinto M., et al. Is zinc-α2-glycoprotein a cardiovascular protective factor for patients undergoing hemodialysis? Clin Chim Acta. 2012 Mar 22;413(5-6):616-9. doi: 10.1016/j.cca.2011.12.002
    Species: Human
    Sample Type: Serum

  25. Gaber E., El Aggan H., El-Banawy H. Glomerular and tubular dysfunctions and their relationship to adiponectin and oxidative stress in obese subjects. Hong Kong Journal of Nephrology Volume 14, Issue 2, October 2012, Pages 38-47
    Species: Human
    Sample Type: Serum

  26. Ari M., Ozturk O., Bez Y., et al. Serum adiponectin and resistin levels in patients with obsessive compulsive disorder. J Affect Disord. 2012 Feb;136(3):979-82. doi: 10.1016/j.jad.2011.07.029.
    Species: Human
    Sample Type: Serum

  27. Tsai H., Chen P., Liu C., et al. Association of hepatitis C virus infection and malnutrition-inflammation complex syndrome in maintenance hemodialysis patients. Nephrol Dial Transplant. 2012 Mar;27(3):1176-83. doi: 10.1093/ndt/gfr458.
    Species: Human
    Sample Type: Serum

  28. Elshamaa M., Sabry S., El-Sonbaty M., et al. Adiponectin: an adipocyte-derived hormone, and its gene encoding in children with chronic kidney disease. BMC Res Notes. 2012 Apr 3;5:174. doi: 10.1186/1756-0500-5-174.
    Species: Human
    Sample Type: Serum

  29. Cuchacovich, Raquel, et al. "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." Clinical rheumatology 30.1 (2011): 133-137.
    Species: Human
    Sample Type: Serum

  30. Hajri, Tahar, et al. "Regulation of adiponectin production by insulin: interactions with tumor necrosis factor-α and interleukin-6." American Journal of Physiology-Endocrinology and Metabolism 300.2 (2011): E350-E360.

  31. Giomisi, Athina, et al. "Serum vaspin levels in normal pregnancy in comparison with non-pregnant women." European journal of endocrinology 164.4 (2011): 579-583.

  32. Kerem M, Ferahkose Z, Yilmaz U, Pasaoglu H, Ofluoglu E, et al. Adipokines and ghrelin in gastric cancer cachexia.World J Gastroenterol. 2008;14(23):3633-3641.
    Species: Human
    Sample Type: Serum

  33. Wang B H, Trayhurn P. Acute and prolonged effects of TNF-α on the expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture. Pflugers Arch - Eur J Physiol. 2006;452: 418-427
    Species: Human
    Sample Type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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