Human Adiponectin ELISA

RayBio® Human Adiponectin (ACRP30) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$756.00  
ELH-Adiponectin-2
+

Assay Format

Detection Range:
25pg/ml - 18,000pg/ml
Recommended Dilution (serum & plasma):
30,000 fold
Sensitivity:
25 pg/ml

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Specificity:
Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q15848
Gene ID:
  • 9370
Gene Symbols:
  • ACDC
  • ACRP30
  • ADIPOQ
  • APM1
  • GBP28
Protein Name & Synonyms:
Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipose most abundant gene transcript 1 protein) (apM-1) (Gelatin-binding protein)
Target Species:
  • Human

Target Background

Adiponectin is a 244-amino acid protein secreted mainly by adipose tissue, but expression is also seen in osteoblasts, liver parenchyma cells, myocytes, epithelial cells, and placental tissue. The biological actions of adiponectin are directed through the receptors AdipoR1, AdipoR2 and T-cadherin. Serum levels of adiponectin decrease with obesity and are positively linked with insulin sensitivity. Low levels of this anti-inflammatory adipokine can produce a chronic state of low-grade inflammation which can lead to insulin resistance, diabetes, hypertension, and atherosclerosis.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Dewulf, Evelyne M., et al. "Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women." Gut (2012): gutjnl-2012.
    Species: Human
    Sample type: Serum (obese women )
  2. Nobili V., Alisi A., Cutrera R., et al. Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver. Thorax. 2015 Aug;70(8):769-81. doi: 10.1136/thoraxjnl-2015-206782
    Species: Human
    Sample type: Serum (Biomarkers in pediatric non-alcoholic fatty liver patients)
  3. Kapil S., Duseja A., Sharma B., et al. Small intestinal bacterial overgrowth and toll-like receptor signaling in patients with non-alcoholic fatty liver disease. J Gastroenterol Hepatol. 2016 Jan;31(1):213-21. doi: 10.1111/jgh.13058.
    Species: Human
    Sample type: Serum (Non-alcoholic fatty acid liver disease patients)
  4. Hoo SP, Loh QL, Yue Z, et al. Preparation of a soft and interconnected macroporous hydroxypropyl cellulose methacrylate scaffold for adipose tissue engineering. J Mater Chem B. 2013 May 20; [Epub ahead of print] doi:10.1039/C3TB00446E
    Species: Human
    Sample type: Conditioned Media (ASCs (adipose-derived stem cells))
  5. Santovito D., Nardis V., Marcantonio P., et al. Plasma exosome microRNA profiling unravels a new potential modulator of adiponectin pathway in Diabetes: effect of glycemic control. The Journal of Clinical Endocrinology & Metabolism 2014 Epub ahead of print June 17 2014, DOI: http://dx.doi.org/10.1210/jc.2013-3843
    Species: Human
    Sample type: Plasma (Naïve diabetic patients)
  6. Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
    Species: Human
    Sample type: Serum (Lupus Nephritis patients)
  7. Volberg V., et al. Associations between perinatal factors and adiponectin and leptin in 9-year-old Mexican-American children. Pediatr Obes. 2013 Dec;8(6):454-63. doi: 10.1111/j.2047-6310.2012.00127.x.
    Species: Human
    Sample type: Plasma (the standard curve was widened)
  8. Hajri, Tahar, et al. "Regulation of adiponectin production by insulin: interactions with tumor necrosis factor-? and interleukin-6." American Journal of Physiology-Endocrinology and Metabolism 300.2 (2011): E350-E360.
    Species: Human
    Sample type: Plasma
  9. Ari M., Ozturk O., Bez Y., et al. Serum adiponectin and resistin levels in patients with obsessive compulsive disorder. J Affect Disord. 2012 Feb;136(3):979-82. doi: 10.1016/j.jad.2011.07.029.
    Species: Human
    Sample type: Serum (Obsessive compulsive disorder patients)
  10. Rathore S., Bidwai A. Association of Insulin Resistance and Selected Adipokines in Cardiovascular Disease with and without Metabolic Syndrome. JMSCR Volume 2, Issue 5, Pages 1176-1186, May 2014
    Species: Human
    Sample type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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