Human Adiponectin ELISA

RayBio® Human Adiponectin (ACRP30) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
25pg/ml - 18,000pg/ml
Recommended Dilution (serum & plasma):
30,000 fold
25 pg/ml

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Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q15848
Gene ID:
  • 9370
Gene Symbols:
  • ACDC
  • ACRP30
  • APM1
  • GBP28
Protein Name & Synonyms:
Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipose most abundant gene transcript 1 protein) (apM-1) (Gelatin-binding protein)
Target Species:
  • Human

Target Background

Adiponectin is a 244-amino acid protein secreted mainly by adipose tissue, but expression is also seen in osteoblasts, liver parenchyma cells, myocytes, epithelial cells, and placental tissue. The biological actions of adiponectin are directed through the receptors AdipoR1, AdipoR2 and T-cadherin. Serum levels of adiponectin decrease with obesity and are positively linked with insulin sensitivity. Low levels of this anti-inflammatory adipokine can produce a chronic state of low-grade inflammation which can lead to insulin resistance, diabetes, hypertension, and atherosclerosis.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. (2020). Mirna 326 Derived Exosomes Is A Potential Modulator Of Adiponectin In Diabetes Mellitus. European Journal of Molecular & Clinical Medicine, 7(6), 2122-2130.
    Species: Human
    Sample type: Plasma
  2. Verônica L.V. Euclydes, Natalia P. Castro, Lourdes R. Lima, Cyro Brito, Laisa Ribeiro, Fernanda Agapito Simões, Guaraci Requena, Liania Alves Luzia, Patricia Helen Rondó, Cord blood concentrations of leptin, zinc-?2-glycoprotein and adiponectin, and adiposity gain in the first three months of life, Nutrition (2018),
    Species: Human
    Sample type: Plasma
  3. Fu, Shi et al. “Lack of adiponectin and adiponectin receptor 1 contributes to benign prostatic hyperplasia.” Oncotarget vol. 8,51 88537-88551. 3 Aug. 2017, doi:10.18632/oncotarget.19877
    Species: Human
    Sample type: Serum
  4. Tenneti, Nikhil, et al. "Concentrations of leptin, adiponectin and other metabolic parameters in non-obese children with Down syndrome." Journal of Pediatric Endocrinology and Metabolism 30.8 (2017): 831-837.
    Species: Human
    Sample type: Plasma
  5. Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
    Species: Human
    Sample type: Serum
  6. Youseef M. The Effect of Dietary Intervention by Low Carbohydrate Diet, and Low Fat Diet, on Weight Loss, Leptin and Adiponectin. Life Science Journal 2015;12(4)
    Species: Human
    Sample type: Serum
  7. Kumar P., Srivastava N., Tiwari S., et al. Correlation of gender and age with serum adipokines level in north indian adults. Asian Journal of Medical Sciences | Sep-Oct 2015 | Vol 6 | Issue 5, 9-11
    Species: Human
    Sample type: Serum
  8. Nabil N., El Sayyad M., et al. moderate exercise training has anorexogenic effect associated with improved oxidative stress in obese women. International Journal of Nutrition and Metabolism. Vol. & (4), pp 52-61, April 2015, DOI: 10.5897/IJNAM2015.0180
    Species: Human
    Sample type: Plasma
  9. Dede N., Ipekci S., Kebapcilar L., et al. Influence of Exercise on Leptin, Adiponectin and Quality of Life in Type 2 Diabetics. Turk J Endocrinol Metab 2015; 19: 7-13
    Species: Human
    Sample type: Serum
  10. Nobili V., Alisi A., Cutrera R., et al. Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver. Thorax. 2015 Aug;70(8):769-81. doi: 10.1136/thoraxjnl-2015-206782
    Species: Human
    Sample type: Serum

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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