Human Allergen Protein Array G2

For simultaneous detection of human IgE and IgG associated with 22 common allergens in animals, mites, fungi (molds), insects, and pollen from trees, grass and weeds..


Assay Format

Species Detected:
  • Human
Compatible Sample Types:
  • Serum
Method of Detection:
Fluorescence Laser Scanner
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

16 Sample Kit

Product Features

  • Simultaneous detection of human IgE and IgG associated with 22 allergen proteins
  • High sensitivity
  • Large dynamic range
  • Low sample volume required (10 - 100 µl per array)
  • Suitable for high-throughput assays
  • High efficiency and accuracy
  • Affordable, quick and simple to use
  • Customizable: Tell us the allergens you're interested in
  • Test 16 samples on each slide

Target Names


Allergen Name

Species - Scientific Name

1Alt a 1Alternaria rot fungus - Alternaria alternata
2Asp f 1Aspergillus fumigatus
3Bla g 5German cockroach - Blattella germanica
4Bla g 6German cockroach - Blattella germanica
5Can f 1Dog - Canis lupus familiaris
6Che a 2Lamb's-quarters, pigweed - Chenopodium album
7Che a 3Lamb's-quarters, pigweed - Chenopodium album
8Cla h 5Cladosporium herbarum mold
9Cla h 8Cladosporium herbarum mold
10Cte f 1Cat flea - Ctenocephalides felis
11Der f 2American house dust mite - Dermatophagoides farinae
12Der p 1European house dust mite - Dermatophagoides pteronyssinus
13Der p 2European house dust mite - Dermatophagoides pteronyssinus
14Fel d 1Cat (saliva) - Felis catus
15Fus c 1Fusarium culmorum
16Gly d 2House itch mite - Glycyphagus domesticus
17Lep d 2Storage mite - Lepidoglyphus destructor
18Mus m 1Mouse - Mus musculus
19Pen c 3Penicillium citrinum
20Pen n 13Penicillium chrysogenum
21Per a 1American cockroach - Periplaneta americana
22Rat n 1Rat (urine) - Rattus norvegicus

Application Notes

Suggested Application
Common allergen detection
Kit Components
  • RayBio® Human Allergen Protein Array G2 Glass Slide
  • RayBio® G series antibody array accessory (including slide incubation chamber assembly, Gasket, Protective cover, Snap-on sides and adhesive film)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotin-conjugated anti-human IgE
  • Biotin-conjugated anti-human IgG
  • Cy3-Conjugated Streptavidin
Other Materials Required
  • Orbital shaker or oscillating rocker
  • Pipettors, pipette tips and other common lab consumables
  • Small plastic boxes or containers
  • Distilled water
  • Aluminum foil
  • Laser scanner for fluorescence detection (compatible laser scanners)
If you do not have access to a compatible laser scanner RayBiotech can provide free scanning service.
Protocol Outline
  1. Sample incubation (1-2 hours)
  2. Biotin-conjugated antibody incubation (1-2 hours)
  3. Cy3-Streptavidin incubation (1 hour)
  4. Scan and data analysis
Use the Analysis Tool for Human Allergen Protein Array G2 to quickly perform data analysis (background subtraction, normalization, etc.).


Upon receipt, all components of the RayBio® Human Allergen Protein Array kit should be stored at -20 °C to -80 °C until just before the experiment. At -20 °C to -80 °C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass chip and Blocking Buffer should be kept at –20 °C and all other component should be stored at 4 °C Use within three months after reagents have been thawed.

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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