Human Allergen Protein Array G1

For simultaneous detection of human IgE and IgG associated with 48 common allergens in human serum samples, including foods, animals, mites, fungi (molds), insects, and pollen from trees, grass and weeds.

$644.63  
PAH-AGN-G1-16
+

Assay Format

Number of Targets Detected:
48
Species Detected:
  • Human
Compatible Sample Types:
  • Serum
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
16 Sample Kit

Product Features

  • Simultaneous detection of human IgE and IgG associated with 48 allergen proteins
  • High sensitivity
  • Large dynamic range
  • Low sample volume required (10 - 100 µl per array)
  • Suitable for high-throughput assays
  • High efficiency and accuracy
  • Affordable, quick and simple to use
  • Customizable: Tell us the allergens you're interested in
  • Test 16 samples on each slide

Target Names

No.

Allergen Name

Species - Scientific Name

1 Act c 1 Kiwi - Actinidia chinensis
2 Act c 2 Kiwi - Actinidia chinensis
3 Alt a 1 Alternaria rot fungus - Alternaria alternata
4 Ana c 2 Pineapple - Ananas comosus
5 Ara h 1 Peanut - Arachis hypogaea
6 Ara h 2 Peanut - Arachis hypogaea
7 Ara h 3 Peanut - Arachis hypogaea
8 Ara h 6 Peanut - Arachis hypogaea
9 Art v 3 Mugwort - Artemisia vulgaris
10 Asp f 1 Aspergillus fumigatus
11 Bla g 5 German cockroach - Blattella germanica
12 Bla g 6 German cockroach - Blattella germanica
13 Bos d 6 Cow (bovine) - Bos taurus
14 Can f 1 Dog (canine) - Canis familiaris
15 Cas s 8 Sweet Chestnut - Castanea sativa
16 Che a 2 Lamb's-quarters - Chenopodium album
17 Che a 3 Lamb's-quarters - Chenopodium album
18 Cla h 5 Mycosphaerella tassiana - Davidiella tassiana (Cladosporium herbarum)
19 Cla h 8 Mycosphaerella tassiana - Davidiella tassiana (Cladosporium herbarum)
20 Cor a 14 European hazelnut - Corylus avellana
21 Cor a 9 European hazelnut - Corylus avellana
22 Cte f 1 Cat flea - Ctenocephalides felis
23 Cuc m 2 Muskmelon - Cucumis melo
24 Der f 2 American house dust mite - Dermatophagoides farinae
25 Der p 1 European house dust mite - Dermatophagoides pteronyssinus
26 Der p 2 European house dust mite - Dermatophagoides pteronyssinus
27 Fel d 1 Cat (feline) - Felis catus
28 Fus c 1 Fusarium culmorum
29 Gly d 2 House itch mite - Glycyphagus domesticus
30 Jug r 1 English walnut - Juglans regia
31 Jug r 2 English walnut - Juglans regia
32 Jug r 3 English walnut - Juglans regia
33 Jug r 4 English walnut - Juglans regia
34 Lep d 2 Fodder mite - Lepidoglyphus destructor
35 Mal d 2 Apple - Malus domestica
36 Mal d 3 Apple - Malus domestica
37 Mus a 4 Banana - Musa acuminata
38 Mus m 1 Mouse - Mus musculus
39 Pen c 3 Penicillium citrinum
40 Pen n 13 Penicillium notatum - Penicillium chrysogenum
41 Per a 1 American cockroach - Periplaneta americana
42 Phl p 1 Common timothy - Phleum pratense
43 Phl p 12 Common timothy - Phleum pratense
44 Pho d 2 Date palm - Phoenix dactylifera
45 Pru p 2.2 Prunus dulcis x Prunus persica
46 Pru p 3 Peach - Prunus persica
47 Rat n 1 Rat - Rattus norvegicus
48 Tri a 14 Wheat - Triticum aestivum

Application Notes

Suggested Application
Common allergen detection
Kit Components
  • RayBio® Human Allergen Protein Array Glass Slide
  • RayBio® G series antibody array accessory (including slide incubation chamber assembly, Gasket, Protective cover, Snap-on sides and adhesive film)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotin-conjugated anti-human IgE
  • Biotin-conjugated anti-human IgG
  • Cy3-Conjugated Streptavidin
Other Materials Required
  • Orbital shaker or oscillating rocker
  • Pipettors, pipette tips and other common lab consumables
  • Small plastic boxes or containers
  • Distilled water
  • Aluminum foil
  • Laser scanner for fluorescence detection (compatible laser scanners)
 
If you do not have access to a compatible laser scanner RayBiotech can provide free scanning service.
Protocol Outline
  1. Sample incubation (1-2 hours)
  2. Biotin-conjugated antibody incubation (1-2 hours)
  3. Cy3-Streptavidin incubation (1 hour)
  4. Scan and data analysis
 
Use the Analysis Tool for Human Allergen Protein Array G1 to quickly perform data analysis (background subtraction, normalization, etc.).

Storage/Stability

Upon receipt, all components of the RayBio® Human Allergen Protein Array kit should be stored at -20 °C to -80 °C until just before the experiment. At -20 °C to -80 °C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass chip and Blocking Buffer should be kept at –20 °C and all other component should be stored at 4 °C Use within three months after reagents have been thawed.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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