RayBio® Human/Mouse/Rat Angiotensin II EIA Kit optimized for serum. Competition-based ELISA on a 96-well strip plate.


Assay Format

Detection Range:
0.1-1,000 pg/ml
Recommended Dilution (serum & plasma):
Human: 4X / Mouse: 2X / Rat: 2X
0.3 pg/ml
This kit can theoretically detect all active angiotensins, including ANGI, ANGII, ANGIII and ANGIV as well as inactive angiotensinogen.

Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, NPY and APC.
Number of Targets Detected:
Species Detected:
  • Human
  • Mouse
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Serum
Design Principle:
  • Competition-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P01019
Gene ID:
  • 183
Gene Symbols:
  • AGT
Protein Name & Synonyms:
Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin-1 (Angiotensin 1-10) (Angiotensin I) (Ang I) Angiotensin-2 (Angiotensin 1-8) (Angiotensin II) (Ang II) Angiotensin-3 (Angiotensin 2-8) (Angiotensin III) (Ang III) (Des-Asp[1]-angiotensin II) Angiotensin-4 (Angiotensin 3-8) (Angiotensin IV) (Ang IV) Angiotensin 1-9 Angiotensin 1-7 Angiotensin 1-5 Angiotensin 1-4]


Angiotensin, a key player in the renin-angiotensin system, is a peptide hormone that causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It is derived from the precursor molecule angiotensinogen produced in the liver.

Angiotensin II is formed from Angiotensin I, which is removed of two terminal residues by the enzyme Angiotensin-converting enzyme (ACE). Angiotensin II acts as an endocrine, autocrine/ paracrine, and intracrine hormone. Angiotensin II is degraded to angiotensin III by angiotensinases that are located in red blood cells and the vascular beds of most tissues. It has a half-life in circulation of around 30 seconds, while in tissue, it may be as long as 15-30 minutes.

The effect of obesity on Angiotensin II has recently been reported. Obese patients show heightened renal vasodilation to blockade of the renin-angiotensin system, suggesting deficits in vascular responses to angiotensin II. This may due to increased reactivity of renal vasoconstriction to ANG II.

Angiotensin II has been associated with a number of important physiological processes in heart, brain, adrenal gland and kidney. For cardiovascular effect, Angiotensin II is a potent direct vasoconstrictor, constricting arteries and veins and increasing blood pressure. It is also the most important Gq stimulator of the heart during hypertrophy. For neural effects, Angiotensin II increases thirst sensation (dipsogen) through the subfornical organ (SFO) of the brain, decreases the response of the baroreceptor reflex, and increases the desire for salt. It increases secretion of ADH in the posterior pituitary and secretion of ACTH in the anterior pituitary. For adrenal effects, Angiotensin II acts on the adrenal cortex, causing it to release aldosterone. For renal effects, Angiotensin II has a direct effect on the proximal tubules to increase Na+ absorption.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  • Prepare all reagents, samples and standards as instructed.
  • Add 100 µl detection antibody to each well.
  • Incubate 1.5 h at RT or O/N at 4°C.
  • Add 100 µl standard or sample to each well.
  • Incubate 2.5 h at RT.
  • Add 100 µl prepared streptavidin solution.
  • Incubate 45 min at RT.
  • Add 100 µl TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl Stop Solution to each well.
  • Read plate at 450 nm immediately.


Standard, Biotinylated Angiotensin II peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
  1. Wang Y., Deb D., Zhang Z., et al. Vitamin D receptor signaling in podocytes protects against diabetic nephropathy. J Am Soc Nephrol. 2012 Dec;23(12):1977-86. doi: 10.1681/ASN.2012040383.
    Species: Mouse
    Sample type: Conditioned Media (Podocyte cell cultures (kidney cells))
  2. Youn J., Zhou J, Cai H. Bone Morphogenic Protein 4 Mediates NOX1-Dependent eNOS Uncoupling, Endothelial Dysfunction, and COX2 Induction in Type 2 Diabetes Mellitus. Mol Endocrinol. 2015 Aug;29(8):1123-33. doi: 10.1210/ME.2014-1313.
    Species: Mouse
    Sample type: Plasma (Endothelial Dysfunction in Type 2 diabetes)
  3. Kong, Juan, et al. "VDR attenuates acute lung injury by blocking Ang-2-Tie-2 pathway and renin-angiotensin system." Molecular endocrinology 27.12 (2013): 2116-2125.
    Species: Mouse
    Sample type: BAL fluid
  4. Sugimura-Wakayama Y., Katafiri W., Osugi M., et al. Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth. Stem Cells Dev. 2015 Nov 15;24(22):2687-99. doi: 10.1089/scd.2015.0104.
    Species: Rat
    Sample type: Plasma (Maternal high fat mice and cardiac dysfunction in offspring)
  5. Mascareno E, Galatioto J, Rozenberg I, et al. Cardiac Lineage Protein-1 (CLP-1) Regulates Cardiac Remodeling via Transcriptional Modulation of Diverse Hypertrophic and Fibrotic Responses and Angiotensin II-transforming Growth Factor ? (TGF-?1) Signaling Axis. The Journal of Biological Chemistry 2012;287(16):13084-13093. doi:10.1074/jbc.M111.288944.
    Species: Mouse
    Sample type: Tissue Lysate (Cardiac left ventricle lysates)
  6. Pandey VG., Jain S., Rana A., et al. Dexamethasone promotes hypertension by allele-specific regulation of the human angiotensinogen gene. J Biol Chem. 2015 Feb 27;290(9):5749-58. doi: 10.1074/jbc.M114.601922.
    Species: Mouse
    Sample type: Plasma
  7. Fouad AA., Al-Mulhim AS., Jresat I. Telmisartan treatment attenuates arsenic-induced hepatotoxicity in mice. Toxicology. 2012 Oct 28;300(3):149-57. doi: 10.1016/j.tox.2012.06.015.
    Species: Mouse
    Sample type: Tissue Lysate (Liver Homogenates)
  8. Xue Q., Chen P., Xiongxiong L., et al. Maternal High-Fat Diet Causes a Sex-Dependent Increase in AGTR2 Expression and Cardiac Dysfunction in Adult Male Rat Offspring. Biol Reprod. 2015 Aug;93(2):49. doi: 10.1095/biolreprod.115.129916
    Species: Rat
    Sample type: Plasma (Maternal high fat diet effects on cardiac function model)
  9. Fouad AA., Al-Mulhim AS., Jresat I., Gomaa W. Therapeutic role of telmisartan against acetaminophen hepatotoxicity in mice. Eur J Pharmacol. 2012 Oct 15;693(1-3):64-71. doi: 10.1016/j.ejphar.2012.07.049.
    Species: Mouse
    Sample type: Tissue Lysate (Liver Tissue lysates in acetaminophen toxicity studies)
  10. Azibani G
    Species: Mouse
    Sample type: Plasma
The kit is great, works very well with human serum which is what I am analyzing. Read instructions very carefully, does have some steps different than any ELISA kit I've used before and the instructions for making up the standards could be clarified some (notate to not put the 450ul of buffer in the highest concentration tube at the beginning of the sentence, not the end) but otherwise a very nice ELISA kit.

University of Rochester
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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