Human Angiogenesis Array C1

RayBio® C-Series Human Angiogenesis Antibody Array 1 Kit. Detects 20 Human Angiogenic Factors. Suitable for all liquid sample types.

$436.00  
AAH-ANG-1-4
+

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Protein Information

Gene Symbols:
ANG | CCL2 | CCL5 | CXCL1 | CXCL2 | CXCL3 | CXCL5 | CXCL8 | EGF | FGF2   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
EGF
ENA-78
(CXCL5)
bFGF
GRO alpha/beta/gamma
IFN-gamma
IGF-1
IL-6
IL-8
(CXCL8)
Leptin
MCP-1
(CCL2)
PDGF-BB
PLGF
RANTES
(CCL5)
TGF beta 1
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
VEGF-A
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Angiogenesis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Casanovas O., Hicklin D., Bergers G., Hanahan D. Drug resistance by evasion of antiangiogenic targeting of VEGF signaling in late-stage pancreatic islet tumors. Cancer Cell . 2005 Oct;8(4):299-309. doi: 10.1016/j.ccr.2005.09.005.
    Species: Human
    Sample type: Tissue Lysate (Whole tumore lysates and Pancreatic islets)
  2. Ye L., Chang Y-H., Xiong Q., et al. Cardiac Repair in a Porcine Model of Acute Myocardial Infarction with Human Induced Pluripotent Stem Cell-Derived Cardiovascular Cells. Cell Stem Cell . 2014 Dec 4;15(6):750-61. doi: 10.1016/j.stem.2014.11.009.
    Species: Human
    Sample type: Conditioned Media (Smooth muscle cells and induced pluoripotent stem cells)
  3. Kuznetsov H., Marsh T., Markens B., et al. Identification of luminal breast cancers that establish a tumor-supportive macroenvironment defined by proangiogenic platelets and bone marrow-derived cells. Cancer Discov. 2012 Dec;2(12):1150-65. doi: 10.1158/2159-8290.CD-12-0216.
    Species: Human
    Sample type: Conditioned Media (Platelet media)
  4. Kuznetsov H., Marsh T., Markens B., et al. Identification of luminal breast cancers that establish a tumor-supportive macroenvironment defined by proangiogenic platelets and bone marrow-derived cells. Cancer Discov. 2012 Dec;2(12):1150-65. doi: 10.1158/2159-8290.CD-12-0216.
    Species: Human
    Sample type: Platelet Releasate (Platelet Releasates)
  5. Dai, Yun, et al. "Disruption of Src function potentiates Chk1-inhibitor-induced apoptosis in human multiple myeloma cells in vitro and in vivo." Blood 117.6 (2011): 1947-1957.
    Species: Human
    Sample type: Conditioned Media
  6. Del Fante, Claudia, et al. "Conditioned Medium Originated From Lethally Irradiated Umbilical Cord Blood-Derived Mononuclear Cells Has Different Pro-Angiogenic Effects Over Mature and Progenitor Endothelial Cells In Vitro." (2013): 1068-1068.
    Species: Human
    Sample type: Conditioned Media (monnuclear cells)
  7. Simcock DE, Kanabar V, Clarke GW, O'Connor BJ, Lee TH, and Hirst SJ. Proangiogenic Activity in Bronchoalveolar Lavage from Patients with Asthma. Am J Respir Crit Care Med 2007; 176(2): 146-153.
    Species: Human
    Sample type: BAL Fluid (Bronchoalveolar lavage fluid (BALF))
  8. Wang D, Wang H, Brown J, Daikoku T, Ning W,et al.CXCL1 induced by prostaglandin E2 promotes angiogenesis in colorectal cancer. J Exp Med. 2006; 203:941-951
    Species: Human
    Sample type: Conditioned Media (conditioned media control cells vs. femal steroid hormone treated)
  9. Xiong Q, Ye L, Zhang P, et al. Bioenergetic and Functional Consequences of Cellular Therapy: Activation of Endogenous Cardiovascular Progenitor Cells. Circulation research 2012;111(4):455-468. doi:10.1161/CIRCRESAHA.112.269894.
    Species: Human
    Sample type: Conditioned Media (Human smooth muscle cells and endothelial cells from hESC cells)
  10. Tang Y, Nakada MT, Kesevan P, McCabe F, et al. Extracellular Matrix Metalloproteinase Inducer Stimulates Tumor Angiogenesis by Elevating Vascular Endothelial Cell Growth Factor and Matrix Metalloproteinases. Cancer Res. 2005;65(8):3193-3199.
    Species: Human
    Sample type: Conditioned Media (Breast carcinoma (MDA-MB-231) cell-conditioned media)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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