Human Angiogenesis Array C1

RayBio® C-Series Human Angiogenesis Antibody Array 1 Kit. Detects 20 Human Angiogenic Factors. Suitable for all liquid sample types.

$435.51  
AAH-ANG-1-4
+

Antigen Information

Gene Symbols:
ANG | CCL2 | CCL5 | CXCL1 | CXCL2 | CXCL3 | CXCL5 | CXCL8 | EGF | FGF2   View more

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
EGF
ENA-78
(CXCL5)
bFGF
GRO alpha/beta/gamma
IFN-gamma
IGF-1
IL-6
IL-8
(CXCL8)
Leptin
MCP-1
(CCL2)
PDGF-BB
PLGF
RANTES
(CCL5)
TGF beta 1
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
VEGF-A
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Angiogenesis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Fosbrink M, Niculescu F, Rus V, Shin ML, Rus H. C5b-9-induced Endothelial Cell Proliferation and Migration Are Dependent on Akt Inactivation of Forkhead Transcription Factor FOXO1. J Biol Chem. 2006; 281(28):19009-19018.
    Species: Human
    Sample type: Conditioned Media
  2. McLachlan E, Shao Q, Wang H, Langlois S, Laird DW. Connexins Act as Tumor Suppressors in Three-dimensional Mammary Cell Organoids by Regulating Differentiation and Angiogenesis. Cancer Res. 2006; 66(20):9886-9894.
    Species: Human
    Sample type: Conditioned Media
  3. Moon MH, Kim SY, Kim YJ, Kim SJ, et al. Human Adipose Tissue-Derived Mesenchymal Stem Cells Improve Postnatal Neovascularization in a Mouse Model of Hindlimb Ischemia. Cell Physiol Biochem. 2006; 17:279-290.
    Species: Human
    Sample type: Conditioned Media
  4. Wang D, Wang H, Brown J, Daikoku T, Ning W,et al.CXCL1 induced by prostaglandin E2 promotes angiogenesis in colorectal cancer. J Exp Med. 2006; 203:941-951
    Species: Human
    Sample type: Conditioned Media
  5. Goldstein LJ, Chen H, Bauer RJ, Bauer SM, Velazquez OC. Normal human fibroblasts enable melanoma cells to induce angiogenesis in type I collagen. Surgery. 2005; 138:439-449.
    Species: Human
    Sample type: Conditioned Media
  6. Tang Y, Nakada MT, Kesevan P, McCabe F, et al. Extracellular Matrix Metalloproteinase Inducer Stimulates Tumor Angiogenesis by Elevating Vascular Endothelial Cell Growth Factor and Matrix Metalloproteinases. Cancer Res. 2005;65(8):3193-3199.
    Species: Human
    Sample type: Conditioned Media
  7. Piwnica D, Touraine P, Struman I, Tabruyn S, et al. Cathepsin D Processes Human Prolactin into Multiple 16K-Like N-Terminal Fragments: Study of their Antiangiogenic Properties and Physiological Relevance. Mol Endocrinol. 2004; 18(10):2522-2542.
    Species: Non-Human Primate
    Sample type: Conditioned Media
  8. Luke?ová S, Kopecký O, Vroblová V, Hlávková D, Andrýs C. Determination of Angiogenic Factors in Serum by Protein Array in Patients with Renal Cell Carcinoma. Folia Biologica (Praha). 2008;54:134-140.
    Species: Human
    Sample type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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