Human Angiogenesis Array C1

RayBio® C-Series Human Angiogenesis Antibody Array 1 Kit. Detects 20 Human Angiogenic Factors. Suitable for all liquid sample types.

$435.51  
AAH-ANG-1-4
+

Antigen Information

Gene Symbols:
ANG | CCL2 | CCL5 | CXCL1 | CXCL2 | CXCL3 | CXCL5 | CXCL8 | EGF | FGF2   View more

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
EGF
ENA-78
(CXCL5)
bFGF
GRO alpha/beta/gamma
IFN-gamma
IGF-1
IL-6
IL-8
(CXCL8)
Leptin
MCP-1
(CCL2)
PDGF-BB
PLGF
RANTES
(CCL5)
TGF beta 1
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
VEGF-A
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Angiogenesis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Fontanilla M., Espinosa L. In vitro and in vivo assessment of oral autologous artificial connective tissue characteristics that influence its performance as a graft. Tissue Eng Part A. 2012 Sep;18(17-18):1857-66. doi: 10.1089/ten.TEA.2011.0421.
    Species: Human
    Sample type: Conditioned Media
  2. Gorantla B., Asuthkar S., Rao J., Patel J., et al. Suppression of the uPAR-uPA System Retards Angiogenesis, Invasion, and In Vivo Tumor Development in Pancreatic Cancer Cells. Molecular Cancer Research. April 2011 9; 377
    Species: Human
    Sample type: Conditioned Media
  3. Chan, Stephen Ho Kin, et al. "The ECM protein LTBP?2 is a suppressor of esophageal squamous cell carcinoma tumor formation but higher tumor expression associates with poor patient outcome."?International journal of cancer?129.3 (2011): 565-573.
    Species: Human
    Sample type: Conditioned Media
  4. Dai, Yun, et al. "Disruption of Src function potentiates Chk1-inhibitor-induced apoptosis in human multiple myeloma cells in vitro and in vivo."?Blood?117.6 (2011): 1947-1957.
    Species: Human
    Sample type: Conditioned Media
  5. van Oorschot, Angelique AM, et al. "Low oxygen tension positively influences cardiomyocyte progenitor cell function."?Journal of cellular and molecular medicine?15.12 (2011): 2723-2734.
    Species: Human
    Sample type: Conditioned Media
  6. Gorantla, Bharathi, et al. "Suppression of the uPAR?uPA system retards angiogenesis, invasion, and in vivo tumor development in pancreatic cancer cells."?Molecular Cancer Research?9.4 (2011): 377-389.
    Species: Human
    Sample type: Conditioned Media
  7. Malla, Rama Rao, et al. "Cathepsin B and uPAR knockdown inhibits tumor-induced angiogenesis by modulating VEGF expression in glioma."?Cancer gene therapy?18.6 (2011): 419-434.
    Species: Human
    Sample type: Conditioned Media
  8. Oskowitz, Adam, et al. "Serum-deprived human multipotent mesenchymal stromal cells (MSCs) are highly angiogenic."?Stem cell research?6.3 (2011): 215-225.
    Species: Human
    Sample type: Conditioned Media
  9. Pang, Xiufeng, et al. "1?-Acetoxychavicol acetate suppresses angiogenesis-mediated human prostate tumor growth by targeting VEGF-mediated Src-FAK-Rho GTPase-signaling pathway."?Carcinogenesis?32.6 (2011): 904-912.
    Species: Human
    Sample type: Conditioned Media
  10. Chan, Matilda F., et al. "Membrane array analysis of tear proteins in ocular cicatricial pemphigoid."?Optometry and vision science: official publication of the American Academy of Optometry?88.8 (2011): 1005.
    Species: Human
    Sample type: Tissue lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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