Human Angiogenesis Array C2

RayBio® C-Series Human Angiogenesis Antibody Array 2 Kit. Detects 23 Human Angiogenic Factors. Suitable for all liquid sample types.

$684.38  
AAH-ANG-2-8
+

Antigen Information

Gene Symbols:
ANGPT1 | ANGPT2 | CCL1 | CCL13 | CCL7 | COL18A1 | CSF2 | CSF3 | CXCL11 | FLT4   View more

Assay Format

Number of Targets Detected:
23
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiopoietin-1
Angiopoietin-2
Angiostatin
Endostatin
GCSF
GM-CSF
I-309
(TCA-3/CCL1)
IL-10
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-4
I-TAC
(CXCL11)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
MMP-1
MMP-9
PECAM-1
(CD31)
Tie-2
TNF alpha
uPAR
VEGFR2
VEGFR3

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Angiogenesis Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Unterman S., Freiman A., Beckerman M., et al. Tuning of collagen scaffold properties modulates embedded endothelial cell regulatory phenotype in repair of vascular injuries in vivo. Adv Healthc Mater. 2015 Oct 28;4(15):2220-8. doi: 10.1002/adhm.201500457.
    Species: Human
    Sample type: Conditioned Media
  2. Rosenzweig D., Gawri R., Krock E., Ouellet J., Stone L., et al. High Mechanical Strain of Intervertebral Disc Cells Promotes Secretion of Inflammatory Factors Associated with Disc Degeneration and Pain. Global Spine Journal, Conference Poster Presentation
    Species: Human
    Sample type: Conditioned Media
  3. Grote, K., et al. "Toll-like receptor 2/6-dependent stimulation of mesenchymal stem cells promotes angiogenesis by paracrine factors."?Eur Cell Mater?26.66 (2013): e79.
    Species: Human
    Sample type: Conditioned Media
  4. Lung H., Cheung A., Ko J., et al Identification of Tumor Suppressor Genes via Cell Fusion and Chromosomal Transfer. Tumor Suppressor Genes, book edited by Yue Cheng, ISBN 978-953-307-879-3, Published: February 3, 2012 DOI: 10.5772/38925
    Species: Human
    Sample type: Conditioned Media
  5. Abajo A., Bitarte N., Zarate R., et al. Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array. World J Gastroenterol. 2012 Feb 21;18(7):637-45. doi: 10.3748/wjg.v18.i7.637.
    Species: Human
    Sample type: Conditioned Media
  6. Li K., Huang Y., Ho C., et al. The role of IL-8 in the SDF-1?/CXCR4-induced angiogenesis of laryngeal and hypopharyngeal squamous cell carcinoma. Oral Oncol. 2012 Jun;48(6):507-15. doi: 10.1016/j.oraloncology.2012.01.006.
    Species: Human
    Sample type: Cell Lysate
  7. Fontanilla M., Espinosa L. In vitro and in vivo assessment of oral autologous artificial connective tissue characteristics that influence its performance as a graft. Tissue Eng Part A. 2012 Sep;18(17-18):1857-66. doi: 10.1089/ten.TEA.2011.0421.
    Species: Human
    Sample type: Conditioned Media
  8. Chan, Matilda F., et al. "Membrane array analysis of tear proteins in ocular cicatricial pemphigoid."?Optometry and vision science: official publication of the American Academy of Optometry?88.8 (2011): 1005.
    Species: Human
    Sample type: Tissue lysate
  9. Chan, Stephen Ho Kin, et al. "The ECM protein LTBP?2 is a suppressor of esophageal squamous cell carcinoma tumor formation but higher tumor expression associates with poor patient outcome."?International journal of cancer?129.3 (2011): 565-573.
    Species: Human
    Sample type: Conditioned Media
  10. van Oorschot, Angelique AM, et al. "Low oxygen tension positively influences cardiomyocyte progenitor cell function."?Journal of cellular and molecular medicine?15.12 (2011): 2723-2734.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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