Human Angiogenesis Array G1000

RayBio® G-Series Human Angiogenesis Antibody Array 1000 Kit. A combination of Human Angiogenesis Antibody Array G1 & G2. Detects 43 Human Angiogenic Factors. Suitable for all liquid sample types.

$1,255.13  
AAH-ANG-G1000-8
+

Assay Format

Number of Targets Detected:
43
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Protein Information

Gene Symbols:
ANG | ANGPT1 | ANGPT2 | CCL1 | CCL13 | CCL2 | CCL5 | CCL7 | COL18A1 | CSF2   View more

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Angiogenin
Angiopoietin-1
Angiopoietin-2
Angiostatin
bFGF
EGF
ENA-78
(CXCL5)
Endostatin
GCSF
GM-CSF
GRO alpha/beta/gamma
I-309
(TCA-3/CCL1)
IFN-gamma
IGF-1
IL-10
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-4
IL-6
IL-8
(CXCL8)
I-TAC
(CXCL11)
Leptin
MCP-1
(CCL2)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
MMP-1
MMP-9
PDGF-BB
PECAM-1
(CD31)
PLGF
RANTES
(CCL5)
TGF beta 1
Thrombopoietin
(TPO)
Tie-2
TIMP-1
TIMP-2
TNF alpha
uPAR
VEGF-A
VEGFR2
VEGFR3
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Angiogenesis Antibody Array G1000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Barcena C., Stefanociv M., Tutusaus A., et al. Angiogenin secretion from hepatoma cells activates hepatic stellate cells to amplify a self-sustained cycle promoting liver cancer. Sci Rep. 2015 Jan 21;5:7916. doi: 10.1038/srep07916.
    Species: Human
    Sample type: Conditioned Media (HepG2, Hep3B, and LX2 cells)
  2. Etulain J., Negrotto S., Tribulatti M., Croci D., Carabelli J., et al. Control of Angiogenesis by Galectins Involves the Release of Platelet-Derived Proangiogenic Factors. PLOS One, Published: April 30, 2014 DOI: 10.1371/journal.pone.0096402 [Epub Ahead of Print]
    Species: Human
    Sample type: Conditioned Media (Human Platelets, cultured media)
  3. Shomer E., Katzenell S., Zipori Y., et al. Microvesicles of pregnant women receiving low molecular weight heparin improve trophoblast function. Thromb Res. 2016 Jan;137:141-7. doi: 10.1016/j.thromres.2015.11.026. Epub 2015 Nov 23.
    Species: Human
    Sample type: Conditioned Media (Microvesicle specimens)
  4. Guo W, Yin G, Liu H, Duan H, Huang Z. Analysis of vascular-associated factors and the prognosis of poorly differentiated hypopharyngeal carcinoma. Oncol Lett. 2020 Nov;20(5):271. doi: 10.3892/ol.2020.12134. Epub 2020 Sep 21. PMID: 32989405; PMCID: PMC7517535.
    Species: Human
    Sample type: Tissue Lysate
  5. Rubio-Azpeitia E., Sanchez P., Delgado D. Three-Dimensional Platelet-Rich Plasma Hydrogel Model to Study Early Tendon Healing. Cells Tissues Organs. 2015;200(6):394-404. doi: 10.1159/000441053.
    Species: Human
    Sample type: Conditioned Media (2d cultures of tendons and platelet rich plasma)
  6. Mamlouk, O. et al. Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes. Int J Radiat Biol. 2012 Dec;88(12):1019-27. doi: 10.3109/09553002.2012.702297. Epub 2012 Jul 9
    Species: Human
    Sample type: Conditioned Media (Colon adenocarcinoma LS174T cells co-culutred with peropheral blood lymphocytes )

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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