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Human Angiogenesis Array Q2

Quantibody® Human Angiogenesis Array 2 Kit. Detects 30 Human Angiogenic Factors. Suitable for all liquid sample types.

Old price: $2,995.20
$2,396.16  
You save: $599.04 (20%)
QAH-ANG-2-4
+

Antigen Information

Gene Symbols:
AGRP | ANG | ANGPT2 | ANGPTL4 | CCL2 | CCL5 | CXCL1 | CXCL10 | CXCL2 | CXCL3   View more

Assay Format

Number of Targets Detected:
30
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Activin A
AgRP
Angiogenin
Angiopoietin-2
ANGPTL4
bFGF
ENA-78
(CXCL5)
GRO alpha/beta/gamma
HB-EGF
HGF
IFN-gamma
IGF-1
IL-1 alpha
(IL-1 F1)
IL-2
IL-6
IL-8
(CXCL8)
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IP-10
(CXCL10)
Leptin
LIF
MCP-1
(CCL2)
PDGF-BB
PLGF
RANTES
(CCL5)
TGF beta 1
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
Thrombopoietin
(TPO)

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Angiogenesis Array Q2 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Das K., et al. Differential expression of steroid 5?-reductase isozymes and association with disease severity and angiogenic genes predict their biological role in prostate cancer. Endocr Relat Cancer. 2010 Aug 16;17(3):757-70. doi: 10.1677/ERC-10-0022.
    Species: Human
    Sample type: Cell Lysate (cell lysate)
  2. Das K., et al. Differential expression of steroid 5?-reductase isozymes and association with disease severity and angiogenic genes predict their biological role in prostate cancer. Endocr Relat Cancer. 2010 Aug 16;17(3):757-70. doi: 10.1677/ERC-10-0022.
    Species: Human
    Sample type:
  3. Xiu, G., Sun, J., Li, X., Jin, H., Zhu, Y., Zhou, X., … Ling, B. (2018). The role of HMGB1 in BMSC transplantation for treating MODS in rats. Cell and Tissue Research, 373(2), 395–406. doi:10.1007/s00441-018-2823-0 
    Species: Human
    Sample type: Conditioned Media (cu;ltured BMSC cells)
  4. Chen J, Liu Z, Hong MM, Zhang H, Chen C, et al. (2014) Proangiogenic Compositions of Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells. PLoS ONE 9(12): e115316. doi:10.1371/journal.pone.0115316
    Species: Human
    Sample type: Cell Lysate (Microvesicles and MSC parents)
  5. Schuster C., Akslen L., Stokowy T., Straume O. Predictive value of angiogenic proteins in patients with metastatic melanoma treated with bevacizumab monotherapy. J Pathol Clin Res. 2019 Jan;5(1):53-62. doi: 10.1002/cjp2.116.
    Species: Human
    Sample type: Serum (Biomarker of angiogenic proteins with metastatic melanoma and bevacizumab)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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