Human Angiostatin ELISA

RayBio® Human Angiostatin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
20 ng/ml - 2000 ng/ml
Recommended Dilution (serum & plasma):
3 - 30 fold
20000 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: human Angiopoietin-1, B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P00747
Gene ID:
  • 5340
Gene Symbols:
  • PLG
Protein Name & Synonyms:
Plasminogen (EC [Cleaved into: Plasmin heavy chain A Activation peptide Angiostatin Plasmin heavy chain A, short form Plasmin light chain B]
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Oh E-T, Park M-T, Song M-J, et al. Radiation-induced angiogenic signaling pathway in endothelial cells obtained from normal and cancer tissue of human breast. Oncogene. 2013 Mar 18; [Epub ahead of print] doi:10.1038/onc.2013.70.
    Species: Human
    Sample type: Conditioned Media (endothelial cells)
  2. Yamahara K., Min KD., Tomoike H., e tal. Heart failure and cardiomyopathy: Pathological role of angiostatin in heart failure: an endogenous inhibitor of mesenchymal stem-cell activation. Heart. 2009 Feb;95(4):283-9. doi: 10.1136/hrt.2008.152223. Epub 2008 Dec 18.
    Species: Human
    Sample type: Serum (Herat failure and control patients)
  3. Navarro-Sobrino, Míriam, et al. "A large screening of angiogenesis biomarkers and their association with neurological outcome after ischemic stroke." Atherosclerosis 216.1 (2011): 205-211.
    Species: Human
    Sample type: Plasma
  4. Pflum ZE, Palumbo SL, Li W-J. Adverse effect of demineralized bone powder on osteogenesis of human mesenchymal stem cells. Exp Cell Res. 2013 Aug 1;319(13):1942-1955.
    Species: Human
    Sample type: Conditioned Media (mesenchymal stem cells)
  5. Wertenbroek MW, Schepers M, Kamminga-Rasker HJ, et al. Clinical outcome, proteome kinetics and angiogenic factors in serum after thermoablation of colorectal liver metastases. BMC Cancer. 2013 May 30;13(1):266.
    Species: Human
    Sample type: Serum
  6. Pei-Huang Wu, Qing-Hua Zhong, Teng-Hui Ma, Qi-Yuan Qin, Xiao-Yan Huang, Ying-Yi Kuang, Huai-Ming Wang, Zi-Xu Yuan, Lei Wang, Dai-Ci Chen, To what extent should the intestinal be resected proximally after radiotherapy: hint from a pathological view, Gastroenterology Report, Volume 8, Issue 4, August 2020, Pages 277–285,
    Species: Human
    Sample type: Tissue Lysate
  7. Sharma, Bal Krishan, et al. "Serum levels of angiogenic and anti-angiogenic factors: their prognostic relevance in locally advanced hepatocellular carcinoma." Molecular and cellular biochemistry 383.1-2 (2013): 103-112.
    Species: Human
    Sample type: Serum (hepatocellular carcinoma)
  8. Park MT., Eun-Taex OH, Min-Jeong SONG, Hyemi LEE and Heon Joo PARK. Radio-sensitivities and angiogenic signaling pathways of irradiated normal endothelial cells derived from diverse human organs. J Radiat Res. 2012 Jul;53(4):570-80. doi: 10.1093/jrr/rrs011
    Species: Human
    Sample type: Conditioned Media (Complete Endothelial cell culture medium endothelial cell growth supplement for HHSECs, human brain microvascular endothelial cells (HBMECs), human ovarian microvascular endothelial cells (HOMECs), human pulmonary endothelial cells (HPM
  9. Mohsen M., et al. Angiogenic output in viral hepatitis, C and B, and HCV-associated hepatocellular carcinoma . Alexandria Journal of Medicine Available online 4 July 2014 In Press, Corrected Proof. DOI: 10.1016/j.ajme.2014.06.003
    Species: Human
    Sample type: Serum
  10. Keskin D., et al. Serum angiostatin levels in patients with Behçet’s disease: does angiogenesis play a role in the pathogenesis of Behçet’s disease? Acta Clinica Belgica Volume 69 Issue 4 (August 2014), pp. 246-250.
    Species: Human
    Sample type: Serum

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